DigiTag assay for multiplex single nucleotide polymorphism typing with high success rate

被引：11

作者：

Nishida, N

Tanabe, T

Hashido, K

Hirayasu, K

Takasu, M

Suyama, A

Tokunaga, K

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Human Genet, Bunkyo Ku, Tokyo 1130033, Japan

[2] Olympus Corp, Biomed Business Incubat Div, Hachioji, Tokyo 1928512, Japan

[3] NovusGene Inc, Div Res & Dev, Hachioji, Tokyo 1928512, Japan

[4] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 346卷 / 02期

关键词：

genotyping method; multiplex genotyping; SNPs; mutation; oligonucleotide ligation assay;

D O I：

10.1016/j.ab.2005.08.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As a consequence of Human Genome Project and single nucleotide polymorphism (SNP) discovery projects, several millions of SNPs, which include possible susceptibility SNPs for multifactorial diseases, have been revealed. Accordingly, there has been a strong drive to perform the investigation with all candidate SNPs for a certain disease without decreasing the number of analyzed SNPs. We developed DigiTag assay, which uses well-designed oligonucleotides called DNA coded numbers (DCNs) in multiplex SNP genotype analysis. During the analysis, the information of a genotype is converted to one of the DCNs in a one to one manner using oligonucleotide ligation assay (encoding). After the encoding reaction, only the DCNs regions and not the SNP specific regions are amplified using the universal primers and then SNP genotype is read out using DNA capillary arrays. DigiTag assay was found to be successful in SNP genotyping, giving a high success rate (24 of 27 SNPs) for randomly chosen SNPs. Moreover, this assay has the potential to analyze almost all kinds of the target SNPs by applying mismatch-induced probes and redesigned primer pairs at a low-cost. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：281 / 288

页数：8

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