Molecular characterization of mtDNA depleted and repleted NT2 cell lines

被引：20

作者：

Binder, DR ^{[1
]}

Dunn, WH ^{[1
]}

Swerdlow, RH ^{[1
]}

机构：

[1] Univ Virginia, Hlth Syst, Dept Neurol, Charlottesville, VA 22908 USA

来源：

MITOCHONDRION | 2005年 / 5卷 / 04期

关键词：

mitochondrial DNA; NT2; cells; transmitochondrial cybrids;

D O I：

10.1016/j.mito.2005.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transmitochondrial cytoplasmic hybrids (cybrids) enable functional assessment of mitochondrial DNA (mtDNA)-encoded proteins. Cybrid production often utilizes cell lines depleted of endogenous mtDNA (p0 cells), and a number of suitable rho 0 cell lines exist for this purpose. We now provide molecular data characterizing an NT2 human teratocarcinoma rho 0 cell line, as well as NT2 cybrid derivatives. NT2 rho 0 cells contained no detectable mtDNA on a sensitive PCR assay. Eight weeks after exogenous mtDNA transfer cybrids showed no evidence of endogenous mtDNA reversion, and heteroplasmic ratios of a single nucleotide substitution roughly reflected that of the blood samples used to repopulate their mtDNA. (c) 2005 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：255 / 265

页数：11

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