Ethyl Acetate Fraction of Amomum villosum var. xanthioides Attenuates Hepatic Endoplasmic Reticulum Stress-Induced Non-Alcoholic Steatohepatitis via Improvement of Antioxidant Capacities

被引:17
作者
Cho, Jung-Hyo [1 ,2 ]
Lee, Jong-Suk [3 ]
Kim, Hyeong-Geug [1 ]
Lee, Hye Won [4 ]
Fang, Zhigang [5 ]
Kwon, Hyeok-Hee [6 ]
Kim, Dong Woon [6 ]
Lee, Chang-Min [7 ]
Jeong, Jin-Woo [7 ]
机构
[1] Indiana Univ, Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA
[2] Daejeon Univ, Dept East & West Canc Ctr, Daejeon Korean Med Hosp, 75,Daedeok Daero 176, Daejeon 35235, South Korea
[3] Gyeonggido Business & Sci Accelerator GBSA, Bioctr, Suwon 16229, South Korea
[4] Korea Inst Oriental Med, Herbal Med Res Div, Daejeon 34054, South Korea
[5] South China Univ Technol, Guangzhou Peoples Hosp 1, Sch Med, Dept Gen Surg, Guangzhou 510180, Peoples R China
[6] Chungnam Natl Univ, Brain Res Inst, Dept Anat & Cell Biol, Coll Med, Daejeon 35015, Peoples R China
[7] Nakdonggang Natl Inst Biol Resources, 137,Donam 2 Gil, Sangju Si 37242, Gyeongsang Buk, Peoples R China
关键词
Amomum villosum var; xanthioides (Wall; ex Baker) T; L; Wu & S; J; Chen; endoplasmic stress; non-alcoholic steatohepatitis; oxidative stress; metallothionein; FATTY LIVER-DISEASE; OXIDATIVE STRESS; ER STRESS; METALLOTHIONEIN; PATHOGENESIS; STEATOSIS; EXPRESSION; XANTHOIDES; INDUCTION; NAFLD;
D O I
10.3390/antiox10070998
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Non-alcoholic fatty liver disease (NAFLD), including non-alcoholic steatohepatitis (NASH), affects 25% of the global population. Despite the prevalence of NAFLD worldwide, effective therapeutics are currently lacking. Amomum villosum var. xanthioides (Wall. ex Baker) T.L.Wu & S.J.Chen (AX) is a medicinal herb traditionally used for treating digestive tract disorders in countries across Asia. We aimed to examine the pharmacological effects of the ethyl acetate fraction of AX (AXEF) against tunicamycin (TM)-induced ER stress in a NASH mouse model using C57/BL6J male mice. Following TM injections (2 mg/kg), the mice were orally administrated AXEF (12.5, 25, or 50 mg/kg), silymarin (50 mg/kg), or distilled water daily for 5 days, and the outcomes for fatty liver, inflammation, and oxidative stress were measured in serum or liver tissue levels. AXEF drastically attenuated hepatic ER stress-induced NASH as indicated by decreases in lipid droplet accumulations, serum liver enzymes, hepatic inflammations, and cell death signals in the hepatic tissue and/or serum levels. Interestingly, AXEF showed potent antioxidant effects by quenching reactive oxidative stress and its final product lipid peroxide in the hepatic tissue, specifically an increase in metallothionein (MT). To confirm the underlying actions of AXEF, we observed that AXEF increases MT1 gene promoter activities in the physiological levels. Collectively, AXEF showed antioxidant properties on TM-induced ER stress in a NASH mice model through the improvement of MTs.
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页数:16
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