Development and Validation of a Sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Screening of Florfenicol and Thiamphenicol in Edible Animal Tissue and Feed

被引：23

作者：

An, Lingling ^{[1
,2
]}

Wang, Yulian ^{[1
,2
]}

Pan, Yuanhu ^{[1
,2
]}

Tao, Yanfei ^{[1
,2
]}

Chen, Dongmei ^{[1
,2
]}

Liu, Zhenli ^{[1
,2
]}

Yang, Wenxiang ^{[3
]}

Peng, Dapeng ^{[1
,2
]}

Yuan, Zonghui ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, MAO Key Lab Detect Vet Drug Residues, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Wuhan 430070, Hubei, Peoples R China

[3] Hubei Prov Ctr Dis Control & Prevent, Wuhan 430079, Hubei, Peoples R China

来源：

FOOD ANALYTICAL METHODS | 2016年 / 9卷 / 09期

关键词：

Florfenicol; Thiamphenicol; Indirect competitive enzyme-linked immunosorbent assay; Edible animal tissue; Feed; PERFORMANCE LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; IMMUNE-RESPONSES; SWINE MUSCLE; AMINE; CHLORAMPHENICOL; FISH; SHRIMP; RESIDUES;

D O I：

10.1007/s12161-016-0436-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To monitor the illegal use of florfenicol (FF) and thiamphenicol (TAP) in edible animal tissue and feed, a sensitive monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed with simple sample preparation and cleanup. The obtained monoclonal antibody (5F4) that has isotype IgG1 showed an IC50 value of 0.21 mu g L-1 for FF and 0.35 mu g L-1 for TAP, respectively. It did not exhibit measurable cross-reactivity with other antibiotics. The limits of detection (LODs) for FF and TAP in a muscle matrix ranged from 0.07 to 0.14 mu g kg(-1) and in a feed matrix ranged from 2.9 to 5.2 mu g kg(-1). The recoveries were 72.8 to 113.4 % with a coefficient of variation of less than 15 %. Good correlation between the ELISA and HPLC-MS/MS results in the tissues tested demonstrated the reliability of our ic-ELISA. This ELISA is a useful tool for screening FF and TAP in edible animal tissue and feed.

引用

页码：2434 / 2443

页数：10

共 31 条

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[Anonymous], 2002, MIN AGR CHIN 235 B

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