Simplified and reliable scheme for species-level identification of Staphylococcus clinical isolates

被引：28
作者：
Iorio, Natalia Lopes Pontes
Ferreira, Rosana Barreto Rocha
Schuenck, Ricardo Pinto
Malvar, Karoline Lourenco
Brilhante, Anike Pereira
Nunes, Ana Paula Ferreira
Bastos, Carla Callegario Reis
dos Santos, Katia Regina Netto
机构：
[1] Univ Fed Rio de Janeiro, Inst Microbiol, BR-21941 Rio De Janeiro, Brazil
[2] Univ Fed Espirito Santo, Dept Pathol, Espirito Santo, Brazil
[3] Marcillo Dias Naval Hosp, Rio De Janeiro, Brazil
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 08期
关键词：
D O I：
10.1128/JCM.00679-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Reliable and rapid identification of staphylococcal strains continues to be a problem faced by many microbiology laboratories. This study evaluates a simplified method that uses a flowchart to assist in the identification of 12 clinical species of Staphylococcus, including eight subspecies. A total of 198 isolates and 11 control strains were identified by the reference method, which employed 22 tests. The results were compared with those obtained by two other methods: an automated system (MicroScan WalkAway) and a simplified method composed of nine tests. The simplified scheme showed an accuracy of 98.5%, while the automated method showed an accuracy of 79.3% (P < 0.001), in identifying staphylococcal species. Atypical phenotypic profiles were detected by both the reference (55.6%) and the simplified (19.7%) methods. The simplified method proposed here was shown to be reliable, with the advantage of being more practical and economic than the reference method.
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页码：2564 / 2569
页数：6
相关论文
共 28 条
[1]  
[Anonymous], 1977, BIOCH TESTS IDENTIFI
[2]   VARIATIONS IN BACITRACIN SUSCEPTIBILITY OBSERVED IN STAPHYLOCOCCUS AND MICROCOCCUS SPECIES [J].
BAKER, JS ;
HACKETT, MF ;
SIMARD, DJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 23 (05) :963-964
[3]   EVALUATION OF THE VITEK SYSTEMS GRAM-POSITIVE IDENTIFICATION CARD FOR SPECIES IDENTIFICATION OF COAGULASE-NEGATIVE STAPHYLOCOCCI [J].
BANNERMAN, TL ;
KLEEMAN, KT ;
KLOOS, WE .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1322-1325
[4]  
Bannerman TL., 2003, MANUAL CLIN MICROBIO, P384
[5]   The changing epidemiology of Staphylococcus aureus? [J].
Chambers, HF .
EMERGING INFECTIOUS DISEASES, 2001, 7 (02) :178-182
[6]   Mucosa or skin as source of coagulase-negative staphylococcal bacteraemia? [J].
Costa, SF ;
Miceli, MH ;
Anaissie, EJ .
LANCET INFECTIOUS DISEASES, 2004, 4 (05) :278-286
[7]  
Cunha MLRS, 2004, MEM I OSWALDO CRUZ, V99, P855, DOI 10.1590/S0074-02762004000800012
[8]   Five-test simple scheme for species-level identification of clinically significant coagulase-negative staphylococci [J].
De Paulis, AN ;
Predari, SC ;
Chazarreta, CD ;
Santoianni, JE .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) :1219-1224
[9]   Survey of infections due to Staphylococcus species:: Frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the SENTRY Antimicrobial Surveillance Program, 1997-1999 [J].
Diekema, DJ ;
Pfaller, MA ;
Schmitz, FJ ;
Smayevsky, J ;
Bell, J ;
Jones, RN ;
Beach, M .
CLINICAL INFECTIOUS DISEASES, 2001, 32 :S114-S132
[10]   Two-center collaborative evaluation of the performance of the BD Phoenix Automated Microbiology System for identification and antimicrobial susceptibility testing of Enterococcus spp. and Staphylococcus spp. [J].
Fahr, AM ;
Eigner, U ;
Armbrust, M ;
Caganic, A ;
Dettori, G ;
Chezzi, C ;
Bertoncini, L ;
Benecchi, M ;
Menozzi, MG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) :1135-1142
123