Affinity Purification of Label-free Tubulins from Xenopus Egg Extracts

被引：7

作者：

Reusch, Sebastian ^{[1
,5
]}

Biswas, Abin ^{[1
,2
]}

Hirst, William Graham ^{[1
,3
]}

Reber, Simone ^{[1
,4
,5
]}

机构：

[1] Humboldt Univ, IRI Life Sci, D-10115 Berlin, Germany

[2] Max Planck Inst Phys Light, D-91058 Erlangen, Germany

[3] Australian Natl Univ, Res Sch Biol, Canberra, ACT 2600, Australia

[4] Univ Appl Sci Berlin, D-13353 Berlin, Germany

[5] Marine Biol Lab, Woods Hole, MA 02543 USA

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 03期

关键词：

MICROTUBULE; CYTOPLASM; XMAP215;

D O I：

10.1016/j.xpro.2020.100151

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post -translational modifications. The protocol is applicable to any cell or tissue of interest.For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).

引用

页数：25

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