Identification of the genetic region of Bacillus subtilis B3 renders Bacillus subtilis 168 biosynthesis of lipopeptide surfactin positive

被引：1

作者：

Yao, SY ^{[1
]}

Gao, XW

Fuchsbauer, N

Hillen, W

Vater, J

Wang, JS

机构：

[1] Nanjing Agr Univ, Minist Agr, Key Lab Monitoring & Management Plant Dis & Insec, Nanjing 210095, Peoples R China

[2] Univ Erlangen Nurnberg, Lehrstuhl Mikrobiol, Inst Mikrobiol Biochem & Genet, D-91058 Erlangen, Germany

[3] Tech Univ Berlin, Inst Chem, Arbeitsgrp Biochem & Mol Biol, D-10587 Berlin, Germany

来源：

BIOCONTROL SCIENCE AND TECHNOLOGY | 2003年 / 13卷 / 07期

关键词：

Bacillus subtilis; phosphopantetheinyl transferase; surfactin; biosynthesis; biological control;

D O I：

10.1080/09583150310001606291

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 3642 base pairs genomic region of Bacillus subtilis B3, which converts B. subtilis 168 into a producer of surfactin, includes srfDB3 encoding thioesterase, aspB3 encoding aspartate aminotransferase, lpaB3 encoding phosphopantetheinyl transferase and yczEB3 encoding YczE-like protein. Deletion analysis and functionally complement results indicated that truncation of srfDB3, aspB3 and yczEB3 did not affect biosynthesis of surfactin in B. subtilis 168. The lpaB3 gene with its promoter and ribosome-binding site is essential, whereas its transcription terminator is not necessary for biosynthesis of surfactin in B. subtilis 168.

引用

页码：691 / 697

页数：7

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