Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

被引：86
作者：
Ha, HC
Juluri, K
Zhou, Y
Leung, S
Hermankova, M
Snyder, SH [1 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA
[3] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA
[4] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 06期
关键词：
D O I：
10.1073/pnas.051633498
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.
引用
收藏
页码：3364 / 3368
页数：5
相关论文
共 53 条
[1]   Identification of poly(ADP-ribose) polymerase as a transcriptional coactivator of the human T-cell leukemia virus type 1 tax protein [J].
Anderson, MG ;
Scoggin, KES ;
Simbulan-Rosenthal, CM ;
Steadman, JA .
JOURNAL OF VIROLOGY, 2000, 74 (05) :2169-2177
[2]   DNA-dependent protein kinase is not required for efficient lentivirus integration [J].
Baekelandt, V ;
Claeys, A ;
Cherepanov, P ;
De Clercq, E ;
De Strooper, B ;
Nuttin, B ;
Debyser, Z .
JOURNAL OF VIROLOGY, 2000, 74 (23) :11278-11285
[3]   Production of high-titer human immunodeficiency virus type 1 pseudotyped with vesicular stomatitis virus glycoprotein [J].
Bartz, SR ;
Vodicka, MA .
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1997, 12 (04) :337-342
[4]   Antisense oligonucleotides: Is the glass half full or half empty? [J].
Bennett, CF .
BIOCHEMICAL PHARMACOLOGY, 1998, 55 (01) :9-19
[5]   SYMPOSIUM - CELLULAR-RESPONSE TO DNA DAMAGE - THE ROLE OF POLY(ADP-RIBOSE) - POLY(ADP-RIBOSE) IN THE CELLULAR-RESPONSE TO DNA DAMAGE [J].
BERGER, NA .
RADIATION RESEARCH, 1985, 101 (01) :4-15
[6]   Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras [J].
Bollag, RJ ;
Crawford, KB ;
Stadt, H ;
Kumiski, D ;
Zdanowicz, M ;
Baptista, C ;
Herlea, V ;
Kirby, ML .
EXPERIMENTAL CELL RESEARCH, 1999, 248 (01) :75-78
[7]   Mice lacking the poly(ADP-ribose) polymerase gene are resistant to pancreatic beta-cell destruction and diabetes development induced by streptozocin [J].
Burkart, V ;
Wang, ZQ ;
Radons, J ;
Heller, B ;
Herceg, Z ;
Stingl, L ;
Wagner, EF ;
Kolb, H .
NATURE MEDICINE, 1999, 5 (03) :314-319
[8]   DNA STRAND BREAKS, NAD METABOLISM, AND PROGRAMMED CELL-DEATH [J].
CARSON, DA ;
SETO, S ;
WASSON, DB ;
CARRERA, CJ .
EXPERIMENTAL CELL RESEARCH, 1986, 164 (02) :273-281
[9]   Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy [J].
Chun, TW ;
Stuyver, L ;
Mizell, SB ;
Ehler, LA ;
Mican, JAM ;
Baseler, M ;
Lloyd, AL ;
Nowak, MA ;
Fauci, AS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) :13193-13197
[10]   INHIBITION OF HIV-1-IIIB REPLICATION IN AA-2 AND MT-2 CELLS IN CULTURE BY 2 LIGANDS OF POLY (ADP-RIBOSE) POLYMERASE - 6-AMINO-1,2-BENZOPYRONE AND 5-IODO-6-AMINO-1,2-BENZOPYRONE [J].
COLE, GA ;
BAUER, G ;
KIRSTEN, E ;
MENDELEYEV, J ;
BAUER, PI ;
BUKI, KG ;
HAKAM, A ;
KUN, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) :504-514
123456