Nitrogen source influences natural abundance 15N of Escherichia coli

被引:16
|
作者
Collins, Jessica G. [1 ]
Dijkstra, Paul [1 ]
Hart, Stephen C. [2 ,3 ]
Hungate, Bruce A. [1 ,3 ]
Flood, Nichole M. [1 ]
Schwartz, Egbert [1 ,3 ]
机构
[1] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA
[2] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA
[3] No Arizona Univ, Merriam Powell Ctr Environm Res, Flagstaff, AZ 86011 USA
关键词
ammonium; natural abundance stable isotope; nitrogen mineralization; nitrogen assimilation;
D O I
10.1111/j.1574-6968.2008.01133.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Escherichia coli cells were forced to mineralize or assimilate nitrogen in vitro by manipulating substrate carbon and nitrogen availability. When grown on an organic nitrogen source, E. coli cells released NH4+ and were enriched in N-15 relative to the nitrogen source (1.6-3.1 parts per thousand). However, when cells were grown on an inorganic nitrogen source, the biomass was depleted (6.1-9.1 parts per thousand) relative to the source. By measuring N-15 enrichment of microorganisms relative to nitrogen pools, ecosystem ecologists may be able to determine if microorganisms are assimilating or mineralizing nitrogen.
引用
收藏
页码:246 / 250
页数:5
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