Bioremediation of an aged hydrocarbon-contaminated soil by a combined system of biostimulation-bioaugmentation with filamentous fungi

被引:109
作者
Mancera-Lopez, M. E. [1 ]
Esparza-Garcia, F. [1 ]
Chavez-Gomez, B. [2 ]
Rodriguez-Vazquez, R. [1 ]
Saucedo-Castaneda, G. [3 ]
Barrera-Cortes, J. [1 ]
机构
[1] CINVESTAV, IPN, Dept Biotecnol & Bioingn, Mexico City 07360, DF, Mexico
[2] Inst Mexicano Petr, Dept Biotecnol, Mexico City 07730, DF, Mexico
[3] Univ Autonoma Metropolitana Iztapalapa, Mexico City 09340, DF, Mexico
关键词
bioaugmentation; bioremediation; hydrocarbons; hydrocarbon-degrading fungi;
D O I
10.1016/j.ibiod.2007.05.012
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This paper presents a study of the effect of it combined biostimulation-bioaugmentation applied to it silty-loam soil polluted with 60,600 mg kg(-1) of it complex mixture of total petroleum hydrocarbons (TPH), which comprises 40% aliphatic hydrocarbons (AH) and 21% polycyclic aromatic hydrocarbons (PAH). The bioaugmentation was performed with Rhizopus sp., Penicillium funiculosum and Aspergillus sydowii strains isolated from two aged soils contaminated with 60,600 and 500,000 mg of TPH per kilogram of dried soil. The native fungi were able to grow in it complex solid mixture of hydrocarbons of high molecular weight, after previous acclimatization in liquid culture. The three fungi mentioned above were able to remove, respectively, 36%, 30% and 17% more PAH in comparison with biostimulation alone. In the bioaugmented systems with Rhizopus sp. and A. sydowii, a positive correlation of respirometric activity (CO2 production) with hydrocarbon removal was obtained (R-2 = 0.75; p(F) = 0.001 and R-2 = 0.78; p(F) = 0.001, respectively); in contrast, P. funiculosum did not show any correlation. An interesting finding from this work is that two of these species of fungi had not previously been reported as being PAH-degrading. (C) 2007 Elsevier Ltd. All rights reserved.
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页码:151 / 160
页数:10
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