RESIST-5 OOKNV and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study

被引：41

作者：

Bianco, G. ^{[1
]}

Boattini, M. ^{[1
]}

van Asten, S. A., V ^{[2
]}

Iannaccone, M. ^{[1
]}

Zanotto, E. ^{[1
]}

Zaccaria, T. ^{[1
]}

Bernards, A. T. ^{[2
]}

Cavallo, R. ^{[1
]}

Costa, C. ^{[1
]}

机构：

[1] Univ Hosp Citta Salute & Sci Torino, Microbiol & Virol Unit, Corso Bramante 88-90, I-10126 Turin, Italy

[2] Leiden Univ, Med Ctr, Dept Med Microbiol, Leiden, Netherlands

来源：

JOURNAL OF HOSPITAL INFECTION | 2020年 / 105卷 / 02期

关键词：

Carbapenemase detection; Enterobacterales; Blood culture; Immunochromatographic assay; Ceftazidime-avibactam resistance; D179Y;

D O I：

10.1016/j.jhin.2020.03.022

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDMand VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture. (C) 2020 Published by Elsevier Ltd on behalf of The Healthcare Infection Society.

引用

页码：162 / 166

页数：5

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