Gradient reversed-phase high-performance chromatography of ionogenic analytes

被引：19

作者：

Kaliszan, Roman ^{[1
]}

Wiczling, Pawel ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Biopharmaceut & Pharmacodynam, PL-80416 Gdansk, Poland

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2011年 / 30卷 / 09期

关键词：

Acidity (pK(a)); Drug; Gradient mode; Hydrophobicity (log k(w)); Ionogenic analyte; Organic modifier; pH gradient; Reversed-phase high-performance liquid chromatography (RP-HPLC); Separation condition; Xenobiotic mixture; NONPOLAR STATIONARY PHASES; LIQUID-CHROMATOGRAPHY; IONIZABLE COMPOUNDS; MOBILE-PHASE; DISSOCIATION-CONSTANTS; ORGANIC MODIFIER; PH VARIATION; RP-HPLC; SIMULTANEOUS-OPTIMIZATION; HYDROPHOBICITY INDEX;

D O I：

10.1016/j.trac.2011.05.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review highlights the basis of gradient reversed-phase high-performance liquid chromatography (RP-HPLC) of ionogenic analytes. We describe pH-gradient RP-HPLC in strict theoretical terms, with examples of experiments to provide improvements in analyte separations and peak shapes, and an original method of pK(a) determination. Finally, we present the concept of the combined pH/organic-modifier gradient mode of RP-HPLC and illustrate it with applications. It allows optimization of separation of ionogenic analytes, along with a method for determining their biorelevant physico-chemical parameters [e.g., hydrophobicity (log k(w)) and acidity (pK(a))]. The method is applicable to drugs and other xenobiotic mixtures, including individual analytes of interest assayed in biological fluids. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1372 / 1381

页数：10

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