Immune response in human chromoblastomycosis and eumycetoma - focusing on human interleukin-17A, interferon-gamma, tumour necrosis factor-alpha, interleukin-1 beta and human beta-defensin-2

被引：4

作者：

Leeyaphan, Charussri ^{[1
,2
]}

Hau, Carren ^{[1
]}

Takeoka, Shintaro ^{[1
]}

Tada, Yayoi ^{[1
]}

Bunyaratavej, Sumanas ^{[2
]}

Pattanaprichakul, Penvadee ^{[2
]}

Sitthinamsuwan, Panitta ^{[3
]}

Chaiprasert, Angkana ^{[4
]}

Sasajima, Yuko ^{[5
]}

Makimura, Koichi ^{[6
]}

Watanabe, Shinichi ^{[1
]}

机构：

[1] Teikyo Univ, Dept Dermatol, Grad Sch Med, Tokyo, Japan

[2] Mahidol Univ, Fac Med, Dept Dermatol, Siriraj Hosp, 2 Wanglang Rd, Bangkok 10700, Thailand

[3] Mahidol Univ, Fac Med, Dept Pathol, Siriraj Hosp, Bangkok, Thailand

[4] Mahidol Univ, Fac Med, Dept Microbiol, Siriraj Hosp, Bangkok, Thailand

[5] Teikyo Univ, Dept Pathol, Grad Sch Med, Tokyo, Japan

[6] Teikyo Univ, Grad Sch Med, Lab Space & Environm Med, Tokyo, Japan

来源：

MYCOSES | 2016年 / 59卷 / 12期

关键词：

Chromoblastomycosis; eumycetoma; immune response; IL-17; human beta-defensin; DIFFERENT CLINICAL FORMS; ANTIMICROBIAL PEPTIDES; EXPRESSION; AGENTS; PROLIFERATION; POLYMORPHISMS; PATHOGENESIS; RECOGNITION; DEFENSE; TISSUES;

D O I：

10.1111/myc.12526

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Knowledge regarding host immune response to chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-), tumour necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1) and human beta-defensin-2 (HBD-2) expressions. Human beta-actin primer was used for confirming DNA detection, and DNA extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin-embedded sections used in this study consisted of five chromoblastomycosis and seven eumycetoma tissues. All PCR reactions showed beta-actin band at 51 bp in all clinical specimens, confirming adequate DNA levels in each reaction. As positive control, the psoriasis skin samples revealed bands for IL-17A at 174 bp, IFN- at 273 bp, TNF- at 360 bp, IL-1 at 276 bp and HBD-2 at 255 bp. For the chromoblastomycosis and eumycetoma tissues, PCR analyses showed IL-17A band at 174 bp in two eumycetoma tissues and HBD-2 band at 255 bp in a chromoblastomycosis tissue. This study demonstrated IL-17A expression in human eumycetoma and HBD-2 expression in human chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.

引用

页码：751 / 756

页数：6

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