From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review

被引：5

作者：

Ramirez-Velasco, Lorena ^{[1
]}

Armendariz-Ruiz, Mariana ^{[1
]}

Alberto Rodriguez-Gonzalez, Jorge ^{[1
]}

Mueller-Santos, Marcelo ^{[2
]}

Asaff-Torres, Ali ^{[3
]}

Carlos Mateos-Diaz, Juan ^{[1
]}

机构：

[1] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Ind Biotechnol, Camino Arenero 1227 El Bajio Arenal, Zapopan, Jalisco, Mexico

[2] Fed State Univ Parana UFPR, Dept Biochem & Mol Biol, Ctr Life Sci, Rua 15 Novembro 1299 Ctr, Curitiba, Parana, Brazil

[3] Ctr Invest Alimentac & Desarrollo AC, Ind Biotechnol, Carretera La Victoria Km 0-6, Hermosillo, Sonora, Mexico

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2016年 / 19卷 / 08期

关键词：

Chromogenic substrates; classical methods; ferulic acid; feruloyl esterases; high throughput screening; hydroxycinnamic esters; PARA-COUMAROYL ESTERASE; PLANT-CELL WALLS; ASPERGILLUS-NIGER; SPECTROPHOTOMETRIC ASSAY; ACID ESTERASES; PURIFICATION; ENZYME; DEGRADATION; SUBSTRATE; DIFFERENTIATION;

D O I：

10.2174/1386207319666151110154722

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Feruloyl esterases (FAEs) are a diverse group of hydrolases widely distributed in plants and microorganisms which catalyzes the cleavage and formation of ester bonds between plant cell wall polysaccharides and phenolic acids. FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of substrates for cleaving ester bonds and synthesizing high-added value molecules through esterification and transesterification reactions. During the past two decades extensive studies have been carried out on the production, characterization and classification of FAEs, however only a few reports of suitable High Throughput Screening assays for this kind of enzymes have been reported. This review is focused on a concise but complete revision of classical to High Throughput Screening methods for FAEs, highlighting its advantages and disadvantages, and finally suggesting future perspectives for this important research field.

引用

页码：616 / 626

页数：11