Replication of Polymer-Based Peptide Microarrays by Multi-Step Transfer

被引：2

作者：

Striffler, Jakob ^{[1
]}

Mattes, Daniela S. ^{[1
,5
]}

Schillo, Sebastian ^{[1
]}

Muenster, Bastian ^{[1
]}

Palermo, Andrea ^{[1
]}

Ridder, Barbara ^{[1
,5
]}

Welle, Alexander ^{[2
,3
]}

Trouillet, Vanessa ^{[3
,4
]}

Stadler, Volker ^{[6
]}

Markovic, Goran ^{[7
]}

Proll, Guenther ^{[7
]}

Braese, Stefan ^{[5
,8
]}

Loeffler, Felix F. ^{[1
]}

Nesterov-Mueller, Alexander ^{[1
]}

Breitling, Frank ^{[1
]}

机构：

[1] KIT, IMT, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[2] KIT, Inst Funct Interfaces IFG, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[3] KIT, KMNF, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[4] KIT, IAM, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

[5] KIT, Inst Organ Chem, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany

[6] PEPperPRINT GmbH, Rischerstr 12, D-69123 Heidelberg, Germany

[7] Univ Tubingen, Morgenstelle 18, D-72076 Tubingen, Germany

[8] KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany

来源：

CHEMNANOMAT | 2016年 / 2卷 / 09期

基金：

欧洲研究理事会;

关键词：

high-throughput screening; label-free detection; peptide array; replication; REFLECTOMETRIC INTERFERENCE SPECTROSCOPY; LABEL-FREE CHARACTERIZATION; SOLID-PHASE SYNTHESIS; SPOT-SYNTHESIS; ARRAYS; PROTEIN; SUPPORT; MEMBRANES; CLEAVAGE; COATINGS;

D O I：

10.1002/cnma.201600194

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peptide microarrays serve as a high-throughput tool for the readout of protein-peptide interactions. We present a multi-step purification of high-complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times.

引用

页码：897 / 903

页数：7

共 7 条

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[7] Multi-step molecular docking and dynamics simulation-based screening of large antiviral specific chemical libraries for identification of Nipah virus glycoprotein inhibitors
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