Using meta-omics of contaminated sediments to monitor changes in pathways relevant to climate regulation

被引:30
作者
Birrer, Simone C. [1 ,2 ]
Dafforn, Katherine A. [3 ]
Sun, Melanie Y. [1 ,2 ,3 ]
Williams, Rohan B. H. [4 ]
Potts, Jaimie [5 ]
Scanes, Peter [5 ]
Kelaher, Brendan P. [6 ,7 ]
Simpson, Stuart L. [8 ]
Kjelleberg, Staffan [4 ,9 ]
Swarup, Sanjay [4 ]
Steinberg, Peter [3 ,9 ]
Johnston, Emma L. [1 ,2 ]
机构
[1] Univ New South Wales, Sch BEES, Evolut & Ecol Res Ctr Is Equivalent, Sydney, NSW 2052, Australia
[2] Sydney Inst Marine Sci, Mosman, NSW 2088, Australia
[3] Macquarie Univ, Dept Environm Sci, N Ryde, NSW 2109, Australia
[4] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551, Singapore
[5] NSW Off Environm & Heritage, Lidcombe, NSW 2141, Australia
[6] Southern Cross Univ, Natl Marine Sci Ctr, Coffs Harbour, NSW 2450, Australia
[7] Southern Cross Univ, Ctr Coastal Biogeochem Res, Coffs Harbour, NSW 2450, Australia
[8] CSIRO Land & Water, Lucas Heights, NSW 2234, Australia
[9] Univ New South Wales, Sch BEES, Ctr Marine Bioinnovat, Sydney, NSW 2052, Australia
基金
澳大利亚研究理事会;
关键词
ANAEROBIC AMMONIUM OXIDATION; DISSIMILATORY NITRATE REDUCTION; MICROBIAL COMMUNITY STRUCTURE; MULTIPLE STRESSORS; SULFIDE INHIBITION; N-2; PRODUCTION; MARINE; DENITRIFICATION; REMOVAL; ANAMMOX;
D O I
10.1111/1462-2920.14470
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbially mediated biogeochemical processes are crucial for climate regulation and may be disrupted by anthropogenic contaminants. To better manage contaminants, we need tools that make real-time causal links between stressors and altered microbial functions, and the potential consequences for ecosystem services such as climate regulation. In a manipulative field experiment, we used metatranscriptomics to investigate the impact of excess organic enrichment and metal contamination on the gene expression of nitrogen and sulfur metabolisms in coastal sediments. Our gene expression data suggest that excess organic enrichment results in (i) higher transcript levels of genes involved in the production of toxic ammonia and hydrogen sulfide and (ii) lower transcript levels associated with the degradation of a greenhouse gas (nitrous oxide). However, metal contamination did not have any significant impact on gene expression. We reveal the genetic mechanisms that may lead to altered productivity and greenhouse gas production in coastal sediments due to anthropogenic contaminants. Our data highlight the applicability of metatranscriptomics as a management tool that provides an immense breadth of information and can identify potentially impacted process measurements that need further investigation.
引用
收藏
页码:389 / 401
页数:13
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