Acetylome and phosphoproteome modifications in imatinib resistant chronic myeloid leukaemia cells treated with valproic acid

被引：15
作者：
Buchi, Francesca [1 ]
Pastorelli, Roberta [2 ]
Ferrari, Germano [1 ]
Spinelli, Elena [1 ]
Gozzini, Antonella [1 ]
Sassolini, Francesca [1 ]
Bosi, Alberto [1 ]
Tombaccini, Donatella [2 ]
Santini, Valeria [1 ]
机构：
[1] Univ Florence, Funct Unit Haematol, AUO Careggi, I-50134 Florence, Italy
[2] Univ Florence, Dept Expt Pathol & Oncol, I-50134 Florence, Italy
来源：
LEUKEMIA RESEARCH | 2011年 / 35卷 / 07期
关键词：
CML BCR/ABL; Proteomics; Imatinib resistance; Valproic acid; Protein phosphorylation; Protein acetylation; CHRONIC MYELOGENOUS LEUKEMIA; HISTONE DEACETYLASE INHIBITORS; TYROSINE KINASE INHIBITOR; BCR-ABL; STEM-CELLS; ANTILEUKEMIA ACTIVITY; K562; CELLS; APOPTOSIS; HEAT-SHOCK-PROTEIN-90; MESYLATE;
D O I：
10.1016/j.leukres.2011.01.033
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Chronic myeloid leukaemia has a specific therapy: BCR/ABL inhibitor imatinib. Resistance due to BCR/ABL dependent and independent mechanisms is partially reversible by histone deacetylase inhibitors. We analysed by 2D-electrophoresis and anti-pan-acetylated and anti-phosphotyrosine immunoblots, followed by spot-matching and MALDI-TOF mass spectrometry, which proteome modifications would parallel restoration of sensitivity to imatinib by valproic acid (VPA). VPA plus imatinib significantly increased acetylation of HSP90 and hnRNP L and decreased phosphorylation of HSPs and hnRNPs in imatinib resistant cells. VPA was able to modify profoundly acetylome and phosphoproteome of CML cells, while reverting resistance to imatinib. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：921 / 931
页数：11
相关论文
共 45 条
  • [1] Imatinib and pegylated human recombinant interferon-α2b in early chronic-phase chronic myeloid leukemia
    Baccarani, M
    Martinelli, G
    Rosti, G
    Trabacchi, E
    Testoni, N
    Bassi, S
    Amabile, M
    Soverini, S
    Castagnetti, F
    Cilloni, D
    Izzo, B
    de Vivo, A
    Messa, E
    Bonifazi, F
    Poerio, A
    Luatti, S
    Giugliano, E
    Alberti, D
    Fincato, G
    Russo, D
    Pane, F
    Saglio, G
    [J]. BLOOD, 2004, 104 (13) : 4245 - 4251
  • [2] Inhibition of histone deacetylase 6 acetylates and disrupts the chaperone function of heat shock protein 90 - A novel basis for antileukemia activity of histone deacetylase inhibitors
    Bali, P
    Pranpat, M
    Bradner, J
    Balasis, M
    Fiskus, W
    Guo, F
    Rocha, K
    Kumaraswamy, S
    Boyapalle, S
    Atadja, P
    Seto, E
    Bhalla, K
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (29) : 26729 - 26734
  • [3] BERAN M, 1993, CANCER RES, V53, P3603
  • [4] Valproic acid stimulates proliferation and self-renewal of hematopoietic stem cells
    Bug, G
    Gül, H
    Schwarz, K
    Pfeifer, H
    Kampfmann, M
    Zheng, XM
    Beissert, T
    Boehrer, S
    Hoelzer, D
    Ottmann, OG
    Ruthardt, M
    [J]. CANCER RESEARCH, 2005, 65 (07) : 2537 - 2541
  • [5] Comparative proteomic analysis of chronic myelogenous leukemia cells: Inside the mechanism of imatinib resistance
    Ferrari, Germano
    Pastorelli, Roberta
    Buchi, Francesca
    Spinelli, Elena
    Gozzini, Antonella
    Bosi, Alberto
    Santini, Valeria
    [J]. JOURNAL OF PROTEOME RESEARCH, 2007, 6 (01) : 367 - 375
  • [6] Cotreatment with vorinostat (Suberoylanilide hydroxamic acid) enhances activity of dasatinib (BMS-354825) against imatinib mesylate-sensitive or imatinib mesylate-resistant chronic myelogenous leukemia cells
    Fiskus, Warren
    Pranpat, Michael
    Balasis, Maria
    Bali, Purva
    Estrella, Veronica
    Kumaraswamy, Sandhya
    Rao, Rekha
    Rocha, Kathy
    Herger, Bryan
    Lee, Francis
    Richon, Victoria
    Bhalla, Kapil
    [J]. CLINICAL CANCER RESEARCH, 2006, 12 (19) : 5869 - 5878
  • [7] Combined effects of novel tyrosine kinase inhibitor AMN107 and histone deacetylase inhibitor LBH589 against Bcr-Abl-expressing human leukemia cells
    Fiskus, Warren
    Pranpat, Michael
    Bali, Purva
    Balasis, Maria
    Kumaraswamy, Sandhya
    Boyapalle, Sandhya
    Rocha, Kathy
    Wu, Jie
    Giles, Francis
    Manley, Paul W.
    Atadja, Peter
    Bhalla, Kapil
    [J]. BLOOD, 2006, 108 (02) : 645 - 652
  • [8] Comparative proteome profiling and functional analysis of chronic myelogenous leukemia cell lines
    Fontana, Simona
    Alessandro, Riccardo
    Barranca, Marilisa
    Giordano, Margherita
    Corrado, Chiara
    Zanella-Cleon, Isabelle
    Becchi, Michel
    Kohn, Elise C.
    De Leo, Giacomo
    [J]. JOURNAL OF PROTEOME RESEARCH, 2007, 6 (11) : 4330 - 4342
  • [9] Molecular mechanisms of resistance to imatinib in Philadelphia-chromosome-positive leukaemias
    Gambacorti-Passerini, CB
    Gunby, RH
    Piazza, R
    Galietta, A
    Rostagno, R
    Scapozza, L
    [J]. LANCET ONCOLOGY, 2003, 4 (02) : 75 - 85
  • [10] GAMBACORTIPASSE.C, 2010, BLOOD, V116, P208
12345