Isolation and characterization of new Puumala orthohantavirus strains from Germany

被引:13
作者
Binder, Florian [1 ]
Reiche, Sven [2 ]
Roman-Sosa, Gleyder [3 ,4 ]
Saathoff, Marion [5 ]
Ryll, Rene [1 ]
Trimpert, Jakob [6 ]
Kunec, Dusan [6 ]
Hoeper, Dirk [3 ]
Ulrich, Rainer G. [1 ,7 ]
机构
[1] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Novel & Emerging Infect Dis, Greifswald, Germany
[2] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Dept Expt Anim Facil & Biorisk Management, Greifswald, Germany
[3] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Diagnost Virol, Greifswald, Germany
[4] Kansas State Univ, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA
[5] Lower Saxony State Off Consumer Protect & Food Sa, Vet Task Force, Specialist Pest Control, Oldenburg, Germany
[6] Free Univ Berlin, Inst Virol, Dept Vet Med, Berlin, Germany
[7] German Ctr Infect Res DZIF, Partner Site Hamburg Lubeck Borstel Insel Riems, Greifswald, Germany
关键词
Puumala orthohantavirus; Bank vole; Cell culture; Virus adaptation; Glycoprotein-specific antibodies; CELL-CULTURE; ENVELOPE GLYCOPROTEINS; COMPLETE GENOME; VIRUS; HANTAVIRUS; ANTIBODIES; IDENTIFICATION; ADAPTATION; RESERVOIR; SEGMENT;
D O I
10.1007/s11262-020-01755-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabruck, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies.
引用
收藏
页码:448 / 460
页数:13
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