Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha)

被引：19
作者：
Donham, RT
Morin, D
Jewell, WT
Lamé, MW
Segall, HJ
Tjeerdema, RS
机构：
[1] Univ Calif Davis, Coll Agr & Environm Sci, Dept Environm Toxicol, Davis, CA 95616 USA
[2] Univ Calif Davis, Sch Vet Med, Dept Mol Biosci, Davis, CA 95616 USA
[3] Univ Calif Davis, Mol Struct Facil, Davis, CA 95616 USA
来源：
AQUATIC TOXICOLOGY | 2005年 / 73卷 / 03期
关键词：
glutathione S-transferases (GST); Chinook salmon; LC-MS/MS; Oncorhynchus tshawytscha;
D O I：
10.1016/j.aquatox.2005.03.012
中图分类号：
Q17 [水生生物学];
学科分类号：
071004 ;
摘要：
Four cytosolic glutathione S-transferase (GST) classes were isolated and characterized from juvenile winter run Chinook salmon (Oncorhynchus tshawytscha) liver. Two techniques were used: (1) gel electrophoresis/immunoblotting against a polyclonal striped bass GST antibody and (2) high-pressure liquid chromatography (HPLC). Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as pi, theta, mu and alpha, by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid (ETHA) were determined to be 0.3 +/- 0.05 U/mg cytosolic protein and 0.06 +/- 0.02 U/mg cytosolic protein, respectively. (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：221 / 229
页数：9
相关论文
共 16 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   THE MAJOR GLUTATHIONE-S-TRANSFERASE IN SALMONID FISH LIVERS IS HOMOLOGOUS TO THE MAMMALIAN PI-CLASS GST [J].
DOMINEY, RJ ;
NIMMO, IA ;
CRONSHAW, AD ;
HAYES, JD .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1991, 100 (01) :93-98
[3]   Characterization of glutathione S-transferases in juvenile white sturgeon [J].
Donham, RT ;
Morin, D ;
Jewell, WT ;
Burns, SA ;
Mitchell, AE ;
Lamé, MW ;
Segall, HJ ;
Tjeerdema, RS .
AQUATIC TOXICOLOGY, 2005, 71 (03) :203-214
[4]   In vitro kinetics of hepatic glutathione S-transferase conjugation in largemouth bass and brown bullheads [J].
Gallagher, EP ;
Sheehy, KM ;
Lame, MW ;
Segall, HJ .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2000, 19 (02) :319-326
[5]   Decreased glutathione S-transferase expression and activity and altered sex steroids in Lake Apopka brown bullheads (Ameriurus nebulosus) [J].
Gallagher, EP ;
Gross, TS ;
Sheehy, KM .
AQUATIC TOXICOLOGY, 2001, 55 (3-4) :223-237
[6]  
George Stephen G., 1994, P37
[7]  
HABIG WH, 1974, J BIOL CHEM, V249, P7130
[8]  
Klaassen C.D., 1996, CASARETT DOULLS TOXI
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   Glutathione turnover is increased during the acute phase of sepsis in rats [J].
Malmezat, T ;
Breuillé, D ;
Capitan, P ;
Mirand, PP ;
Obled, C .
JOURNAL OF NUTRITION, 2000, 130 (05) :1239-1246
12