Electrochemical sensor for guanine using a self-assembled monolayer of 1,8,15,22-tetraaminophthalocyanatonickel(II) on glassy carbon electrode

被引：18

作者：

Jeevagan, A. John ^{[1
]}

John, S. Abraham ^{[1
]}

机构：

[1] Gandhigram Rural Inst, Dept Chem, Gandhigram 624302, Dindigul, India

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 424卷 / 01期

关键词：

Self-assembled monolayer; 4 alpha-Tetraaminophthalocyanatonickel(II); Guanine; Adenine; Cyclic voltammetry; CAPILLARY-ZONE-ELECTROPHORESIS; PYROLYTIC-GRAPHITE ELECTRODE; TIN OXIDE ELECTRODE; ELECTROCATALYTIC OXIDATION; BIOLOGICAL-FLUIDS; PHARMACEUTICAL FORMULATIONS; VOLTAMMETRIC DETERMINATION; AMPEROMETRIC DETECTION; METAL PHTHALOCYANINES; COBALT PHTHALOCYANINE;

D O I：

10.1016/j.ab.2012.02.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes the selective determination of guanine (G) using the self-assembled monolayer (SAM) of 1,8,15,22-tetraaminophthalocyanatonickel(II) (4 alpha-N(II)TAPc) modified glassy carbon electrode (GCE) in 0.2 M acetate buffer solution (pH 4.0). The SAM of 4 alpha-Ni(II)TAPc was formed on GCE by spontaneous adsorption of 1 mM 4 alpha-Ni(II)TAPc in dimethylformamide (DMF). It shows two pairs of redox waves corresponding to Ni-III/Ni-II and (NiPc-1)-Pc-III/(NiPc-2)-Pc-III in 0.2 M acetate buffer solution. The SAM modified electrode exhibits excellent electrocatalytic activity toward the oxidation of G by enhancing its oxidation current with 150 mV less positive potential shift in contrast to bare GCE. Furthermore, the SAM modified electrode selectively determines G in the presence of high concentration of adenine (A). In differential pulse voltammetry measurements, the oxidation current response of G was increased linearly in the concentration range of 10 to 100 mu M, and a detection limit was found to be 3 x 10(-8) M (signal/noise = 3). (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：21 / 26

页数：6