Development, Optimization and Validation of an HPLC-PDA Method for Quantification of Taxifolin in the Bark Extract of Pinus pinaster

被引:1
|
作者
de Almeida, Priscila A. [1 ]
Bhering, Carla A. P. [1 ]
Alves, Michele C. [1 ]
de Oliveira, Marcone A. L. [2 ]
Raposo, Nadia R. B. [1 ]
Ferreira, Anderson O. [3 ]
Brandao, Marcos A. F. [1 ]
机构
[1] Univ Fed Juiz de Fora, Nucleo Pesquisa & Inovacao Ciencias Saude NUPICS, BR-36036900 Juiz De Fora, MG, Brazil
[2] Univ Fed Juiz de Fora, GQAQ, BR-36036900 Juiz De Fora, MG, Brazil
[3] Ortofarma, Lab Controle Qualidade, BR-36120000 Matias Barbosa, MG, Brazil
关键词
analytical validation; high performance liquid chromatography; Pinus pinaster; quality control; taxifolin; CAPILLARY-ZONE-ELECTROPHORESIS; MASS-SPECTROMETRY; POLYPHENOLS; DIHYDROQUERCETIN; PYCNOGENOL(R); STRESS; FOOD;
D O I
10.5935/0103-5053.20160044
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The aqueous extract of the bark of Pinus pinaster has a high concentration of polyphenols represented by a mixture of procyanidins, besides taxifolin, phenolic acids, cinnamic acids and their glycosides. Its quality control is specified in the United States Pharmacopeia, and the assay test is performed by determination of the total procyanidins content. However, determining the individual polyphenol content may represent an additional quality parameter for this extract. In this sense, the present study aimed to develop and optimize a method of high performance liquid chromatography with photodiode array detection (HPLC-PDA) for quantification of taxifolin in the bark extract of P. pinaster, using a 3(3) Box-Behnken factorial design. The proposed method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness and it has shown that taxifolin may be used as a chemical marker for quality control of the bark extract of P. pinaster.
引用
收藏
页码:1648 / 1656
页数:9
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