The aqueous extract of the bark of Pinus pinaster has a high concentration of polyphenols represented by a mixture of procyanidins, besides taxifolin, phenolic acids, cinnamic acids and their glycosides. Its quality control is specified in the United States Pharmacopeia, and the assay test is performed by determination of the total procyanidins content. However, determining the individual polyphenol content may represent an additional quality parameter for this extract. In this sense, the present study aimed to develop and optimize a method of high performance liquid chromatography with photodiode array detection (HPLC-PDA) for quantification of taxifolin in the bark extract of P. pinaster, using a 3(3) Box-Behnken factorial design. The proposed method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness and it has shown that taxifolin may be used as a chemical marker for quality control of the bark extract of P. pinaster.
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Korea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South KoreaKorea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South Korea
Shim, Ji-Won
Kim, Sun-Hae
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Korea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South KoreaKorea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South Korea
Kim, Sun-Hae
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Shin, Han-Seung
Shin, Jae-Wook
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Korea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South KoreaKorea Adv Food Res Inst, 50 Botdeul Ro, Uiwang Si 16001, Gyeonggi Do, South Korea
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Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
Pramod K.
Ilyas U.K.
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Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
Ilyas U.K.
Kamal Y.T.
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Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
Kamal Y.T.
Ahmad S.
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Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
Ahmad S.
Ansari S.H.
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Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
Ansari S.H.
Ali J.
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Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New DelhiDepartment of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi
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Korea Inst Sci & Technol, Ctr Adv Biomol Recognit, Seoul 02792, South KoreaKorea Inst Sci & Technol, Ctr Adv Biomol Recognit, Seoul 02792, South Korea
Kim, Hyoung Ja
Lee, Jae Yeol
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Kyung Hee Univ, Res Inst Basic Sci, Seoul 02447, South Korea
Kyung Hee Univ, Coll Sci, Dept Chem, Seoul 02447, South KoreaKorea Inst Sci & Technol, Ctr Adv Biomol Recognit, Seoul 02792, South Korea
Lee, Jae Yeol
Cheon, Chunhoo
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Kyung Hee Univ, Coll Korean Med, Dept Prevent Med, Seoul 02447, South KoreaKorea Inst Sci & Technol, Ctr Adv Biomol Recognit, Seoul 02792, South Korea
Cheon, Chunhoo
Ko, Seong-Gyu
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Kyung Hee Univ, Coll Korean Med, Dept Prevent Med, Seoul 02447, South KoreaKorea Inst Sci & Technol, Ctr Adv Biomol Recognit, Seoul 02792, South Korea
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Chung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South KoreaChung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South Korea
Jeong, Yujin
Lee, Sukjin
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Chung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South KoreaChung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South Korea
Lee, Sukjin
Whang, Wankyunn
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Chung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South Korea
Chung Ang Univ, Seoul 06974, South KoreaChung Ang Univ, Dept Global Innovat Drugs, Coll Pharm, Grad Sch, Seoul, South Korea