Human herpesvirus-6 infection: A prospective study evaluating HHV-6 DNA levels in liver from children with acute liver failure

被引：30

作者：

Chevret, Laurent ^{[1
]}

Boutolleau, David ^{[2
,6
]}

Halimi-Idri, Nadia ^{[2
]}

Branchereau, Sophie ^{[7
]}

Baujard, Catherine ^{[3
]}

Fabre, Monique ^{[4
]}

Gautheret-Dejean, Agnes ^{[6
]}

Debray, Dominique ^{[5
]}

机构：

[1] CHU Bicetre, AP HP, Pediat Intens Care Unit, F-94275 Le Kremlin Bicetre, France

[2] CHU Bicetre, AP HP, Virol Lab, Le Kremlin Bicetre, France

[3] CHU Bicetre, AP HP, Pediat Anesthesiol Unit, Le Kremlin Bicetre, France

[4] CHU Bicetre, AP HP, Anatomopathol Unit, Le Kremlin Bicetre, France

[5] CHU Bicetre, AP HP, Pediat Hepatol Unit, Le Kremlin Bicetre, France

[6] Hop La Pitie Salpetriere, AP HP, Virol Lab, Paris, France

[7] CHU Bicetre, AP HP, Pediat Surg, Le Kremlin Bicetre, France

来源：

JOURNAL OF MEDICAL VIROLOGY | 2008年 / 80卷 / 06期

关键词：

human herpesvirus 6; acute liver failure; diagnosis; real-time PCR; children;

D O I：

10.1002/jmv.21143

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this prospective study was to investigate the role of HHV-6 infection in children with acute onset of liver failure using real-time quantitative PCR. Twenty-three children (median age, 24 months) were included: 6 cases of fulminant hepatic failure of undetermined cause (group 1); 4 cases of fulminant hepatic failure of recognized cause (group 2); 3 cases of acute decompensation of chronic liver disease (group 3); and 10 cases of chronic liver disease (group 4). HHV-6 genomic DNA was detected and quantified using real-time PCR in plasma and livers obtained at the time of transplantation. HHV6-DNA detection rate was significantly higher among groups 1, 2, and 3 compared to group 4 (76.9% vs. 20% P=0.02). Viral loads ranged from 6 to 32,500 copies/106 cells. Significantly higher viral loads were found in 4 of 9 children with acute onset of liver failure of unknown origin (group 1, n = 3; group 3, n = 1) and 1 child with fulminant autoimmune hepatitis (group 2) (P=0.03). These results strongly support the hypothesis that HHV-6 may cause fulminant hepatic failure and acute decompensation of chronic liver disease in children. Nevertheless, a threshold viral load value still remains to be determined.

引用

页码：1051 / 1057

页数：7

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