Neoplastic transformation and induction of H+,K+-adenosine triphosphatase by N-methyl-N′-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line

被引：44

作者：

Shimokawa, Osamu ^{[1
]}

Matsui, Hirofumi ^{[1
]}

Nagano, Yumiko ^{[1
]}

Kaneko, Tsuyoshi ^{[1
]}

Shibahara, Takeshi ^{[1
]}

Nakahara, Akira ^{[1
]}

Hyodo, Ichinosuke ^{[1
]}

Yanaka, Akinori ^{[2
]}

Majima, Hideyuki J. ^{[3
,4
]}

Nakamura, Yukio ^{[5
]}

Matsuzaki, Yasushi ^{[6
]}

机构：

[1] Univ Tsukuba, Div Gastroenterol, Grad Sch Comprehens Human Sci, Tsukuba, Ibaraki 3058575, Japan

[2] Tokyo Univ Sci, Fac Pharmaceut Sci, Chiba, Japan

[3] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Oncol, Kagoshima 890, Japan

[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Environm Med, Kagoshima 890, Japan

[5] RIKEN, BioResource Ctr, Cell Engn Div, Dept Biol Syst, Tsukuba, Ibaraki, Japan

[6] Tokyo Med Univ, Kasumigaura Hosp, Div Gastroenterol, Ibaraki, Japan

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2008年 / 44卷 / 1-2期

关键词：

RGM-1; H+; K+-adenosine triphosphatase; (H+; K+-ATPase); neoplastic transformation; N-methyl-N '-nitro-N-nitrosoguanidine (MNNG);

D O I：

10.1007/s11626-007-9067-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induces gastric cancer in animal models. We established an MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line, which we named RGK-1, that could be used as an in vitro model of gastric cancer. This cell line showed signs of neoplasia and transformation, in that it lost contact inhibition and formed tumors in nude mice. The mutant cells also expressed parietal cell-specific H+,K+-adenosine triphosphatase (H+,K+-ATPase), which parent RGM-1 did not. The results suggested that parent RGM-1 cells were gastric progenitor cells. This mutant RGK-1 cell line will contribute to future investigation on gastric carcinogenesis and to the development of other pathophysiologic fields.

引用

页码：26 / 30

页数：5

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