Comparison between Effects of Free Curcumin and Curcumin Loaded NIPAAm-MAA Nanoparticles on Telomerase and PinX1 Gene Expression in Lung Cancer Cells

被引：49

作者：

Badrzadeh, Fariba ^{[1
,2
]}

Akbarzadeh, Abolfazl ^{[1
,4
]}

Zarghami, Nosratollah ^{[1
,3
]}

Yamchi, Mohammad Rahmati ^{[1
,3
]}

Zeighamian, Vahide ^{[1
]}

Tabatabae, Fateme Sadate ^{[1
]}

Taheri, Morteza ^{[1
]}

Kafil, Hossein Samadi ^{[5
]}

机构：

[1] Tabriz Univ Med Sci, Dept Med Biotechnol, Fac Adv Med Sci, Tabriz, Iran

[2] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[3] Tabriz Univ Med Sci, Dept Clin Biochem, Fac Med, Tabriz, Iran

[4] Tabriz Univ Med Sci, Dept Med Nanotechnol, Fac Adv Med Sci, Tabriz, Iran

[5] Tabriz Univ Med Sci, Fac Med Sci, Dept Drug Appl Res Ctr, Tabriz, Iran

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2014年 / 15卷 / 20期

关键词：

Curcumin; NIPAAm-MAA; PinX1; telomerase; lung cancer cells; HUMAN BREAST-CANCER; RISK-FACTORS; PH; PROLIFERATION; PACLITAXEL;

D O I：

10.7314/APJCP.2014.15.20.8931

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Herbal compounds such as curcumin which decrease telomerase and gene expression have been considered as beneficial tools for lung cancer treatment. In this article, we compared the effects of pure curcumin and curcumin-loaded NIPAAm-MAA nanoparticles on telomerase and PinX1 gene expression in a lung cancer cell line. Materials and Methods: A tetrazolium-based assay was used for determination of cytotoxic effects of curcumin on the Calu-6 lung cancer cell line and telomerase and pinX1 gene expression was measured with real-time PCR. Results: MTT assay showed that Curcumin-loaded NIPAAm-MAA inhibited the growth of the Calu-6 lung cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of curcumin-loaded NIPAAm-MAA increased while expression of the PinX1 gene became elevated. Conclusions: The results showed that curcumin-loaded-NIPAAm-MAA exerted cytotoxic effects on the Calu-6 cell line through down-regulation of telomerase and stimulation of pinX1 gene expression. NIPPAm-MAA could be good carrier for such kinds of hydrophobic agent.

引用

页码：8931 / 8936

页数：6

共 21 条

[1] Curcumin suppresses the paclitaxel-induced nuclear factor-κB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice [J].