A simple bioluminescent method for measuring D-amino acid oxidase activity

被引：15

作者：

Bailey, T. Spencer ^{[1
]}

Donor, Micah T. ^{[1
]}

Naughton, Sean P. ^{[1
]}

Pluth, Michael D. ^{[1
]}

机构：

[1] Univ Oregon, Inst Mol Biol, Inst Mat Sci, Dept Chem & Biochem, Eugene, OR 97403 USA

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 25期

关键词：

HYDROGEN-SULFIDE; IN-VIVO; PEROXIDE; SULFHYDRATION; CYSTEINE; RELEASE; BRAIN; CELLS; MODEL;

D O I：

10.1039/c4cc08145e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

D-Amino acid oxidase (DAO) plays important roles in regulating D-amino acid neurotransmitters and was recently identified as a key enzyme integral to hydrogen sulfide production from D-Cys. We report here the development of a simple biocompatible, bioluminescent method for measuring DAO activity based on the highly selective condensation of D-Cys with 6-hydroxy-2-cyanobenzothiazole (CBT-OH) to form D-luciferin.

引用

页码：5425 / 5428

页数：4

共 19 条

[1] Understanding Hydrogen Sulfide Storage: Probing Conditions for Sulfide Release from Hydrodisulfides [J].

Bailey, T. Spencer ;

Zalcharov, Lev N. ;

Pluth, Michael D. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (30) :10573-10576

[2] Copper-free click chemistry for dynamic in vivo imaging [J].

Baskin, Jeremy M. ;

Prescher, Jennifer A. ;

Laughlin, Scott T. ;

Agard, Nicholas J. ;

Chang, Pamela V. ;

Miller, Isaac A. ;

Lo, Anderson ;

Codelli, Julian A. ;

Bertozzi, Carolyn R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (43) :16793-16797

[3] Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling [J].

Ida, Tomoaki ;

Sawa, Tomohiro ;

Ihara, Hideshi ;

Tsuchiya, Yukihiro ;

Watanabe, Yasuo ;

Kumagai, Yoshito ;

Suematsu, Makoto ;

Motohashi, Hozumi ;

Fujii, Shigemoto ;

Matsunaga, Tetsuro ;

Yamamoto, Masayuki ;

Ono, Katsuhiko ;

Devarie-Baez, Nelmi O. ;

Xian, Ming ;

Fukuto, Jon M. ;

Akaike, Takaaki .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (21) :7606-7611

[4]

Ishigami M, 2009, ANTIOXID REDOX SIGN, V11, P205, DOI 10.1089/ARS.2008.2132

[5] Polysulfides are possible H2S-derived signaling molecules in rat brain [J].

Kimura, Yuka ;

Mikami, Yoshinori ;

Osumi, Kimiko ;

Tsugane, Mamiko ;

Oka, Jun-ichiro ;

Kimura, Hideo .

FASEB JOURNAL, 2013, 27 (06) :2451-2457

[6] Polysulfide exerts a protective effect against cytotoxicity caused by t-buthylhydroperoxide through Nrf2 signaling in neuroblastoma cells [J].

Koike, Shin ;

Ogasawara, Yuki ;

Shibuya, Norihiro ;

Kimura, Hideo ;

Ishii, Kazuyuki .

FEBS LETTERS, 2013, 587 (21) :3548-3555

[7] H2S Signals Through Protein S-Sulfhydration [J].

Mustafa, Asif K. ;

Gadalla, Moataz M. ;

Sen, Nilkantha ;

Kim, Seyun ;

Mu, Weitong ;

Gazi, Sadia K. ;

Barrow, Roxanne K. ;

Yang, Guangdong ;

Wang, Rui ;

Snyder, Solomon H. .

SCIENCE SIGNALING, 2009, 2 (96) :ra72

[8] Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation [J].

Nguyen, Duy P. ;

Elliott, Thomas ;

Holt, Matthew ;

Muir, Tom W. ;

Chin, Jason W. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (30) :11418-11421

[9] Physiological functions of D-amino acid oxidases: from yeast to humans [J].

Pollegioni, L. ;

Piubelli, L. ;

Sacchi, S. ;

Pilone, M. S. ;

Molla, G. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2007, 64 (11) :1373-1394

[10]

Ren H, 2009, ANGEW CHEM, V121, P9838

← 1 2 →