Molecular cloning and functional characterization of a thioredoxin peroxidase gene in Echinococcus multilocularis

被引：0
作者：
Guo, Xiaola [1 ,2 ]
Zhang, Junmei [1 ]
Li, Rui [1 ]
Li, Hong [1 ]
He, Xuedong [1 ]
Wang, Shuai [1 ]
Wang, Zhengrong [2 ]
Harandi, Majid Fasihi [3 ]
Hu, Junjie [4 ]
Zheng, Yadong [1 ,5 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China
[2] Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China
[3] Kerman Univ Med Sci, Res Ctr Hydatid Dis Iran, Dept Parasitol, Kerman, Iran
[4] Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China
[5] Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2021年 / 245卷
基金：
中国国家自然科学基金;
关键词：
Echinococcus multilocularis; Thioredoxin peroxidase; Peroxidase activity; Oxidative stress; M2; macrophage; 2-CYS PEROXIREDOXINS; EXPRESSION; FALCIPARUM; MECHANISMS; ALVEOLAR; FOXES;
D O I：
10.1016/j.molbiopara.2021.111408
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thioredoxin peroxidase (TPx) plays an important role in protecting parasites against oxidative damage. However, studies on the role of TPxs in Echinococcus multilocularis are limited. In this study, one tpx gene of E. multilocularis, named as emtpx-1, was identified. EmTPx-1 shares two positionally conserved cysteine residues (Cys48 and Cys169) with orthologs from other platyhelminths. EmTPx-1 is highly expressed in the germinal layer and present in exosome-like vesicles secreted by E. multilocularis metacestodes. EmTPx-1 displays peroxidase activity, which removes hydrogen peroxide in the presence of dithiothreitol. Furthermore, EmTPx-1 could protect DNA from oxidative damages, and EmTPx-1-expressing E. coli cells had an enhanced resistance to oxidative stress. In addition, EmTPx-1 enhanced the expression of arg1, ym1, and il-10, but suppressed inos, tnf-alpha, and il-1 beta expression in LPS-stimulated macrophages. Our data suggest a critical role for EmTPx-1 in oxidative stresses and M2 macrophage polarization.
引用
收藏
页数：7
相关论文
共 36 条
  • [1] Oxidative Stress and Antioxidant Defense
    Birben, Esra
    Sahiner, Umit Murat
    Sackesen, Cansin
    Erzurum, Serpil
    Kalayci, Omer
    [J]. WORLD ALLERGY ORGANIZATION JOURNAL, 2012, 5 : 9 - 19
  • [2] Release of reactive oxygen species by phagocytic cells in response to live parasites in mice infected with Trypanosoma cruzi
    Cardoni, RL
    Antunez, MI
    Morales, C
    Nantes, IR
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (03) : 329 - 334
  • [3] Echinococcus multilocularis
    Casulli, Adriano
    Barth, Thomas F. E.
    Tamarozzi, Francesca
    [J]. TRENDS IN PARASITOLOGY, 2019, 35 (09) : 738 - 739
  • [4] Molecular cloning and characterization of two genes encoding 2-Cys peroxiredoxins from Fasciola gigantica
    Chaithirayanon, Kulathida
    Sobhon, Prasert
    [J]. EXPERIMENTAL PARASITOLOGY, 2010, 125 (02) : 106 - 113
  • [5] Global Distribution of Alveolar and Cystic Echinococcosis
    Deplazes, P.
    Rinaldi, L.
    Rojas, C. A. Alvarez
    Torgerson, P. R.
    Harandi, M. F.
    Romig, T.
    Antolova, D.
    Schurer, J. M.
    Lahmar, S.
    Cringoli, G.
    Magambo, J.
    Thompson, R. C. A.
    Jenkins, E. J.
    [J]. ECHINOCOCCUS AND ECHINOCOCCOSIS, PT A, 2017, 95 : 315 - 493
  • [6] GENERATION OF REACTIVE OXYGEN SPECIES IN WHOLE-BLOOD FROM PATIENTS WITH ACUTE FALCIPARUM-MALARIA
    DESCAMPSLATSCHA, B
    LUNELFABIANI, F
    KARABINIS, A
    DRUILHE, P
    [J]. PARASITE IMMUNOLOGY, 1987, 9 (02) : 275 - 279
  • [7] Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages
    Donnelly, S
    O'Neill, SM
    Sekiya, M
    Mulcahy, G
    Dalton, JP
    [J]. INFECTION AND IMMUNITY, 2005, 73 (01) : 166 - 173
  • [8] The innate allergenicity of helminth parasites
    Falcone, FH
    Loukas, A
    Quinnell, RJ
    Pritchard, DI
    [J]. CLINICAL REVIEWS IN ALLERGY & IMMUNOLOGY, 2004, 26 (01) : 61 - 72
  • [9] Threat of alveolar echinococcosis to public health - a challenge for Europe
    Gottstein, Bruno
    Stojkovic, Marija
    Vuitton, Dominique A.
    Millon, Laurence
    Marcinkute, Audrone
    Deplazes, Peter
    [J]. TRENDS IN PARASITOLOGY, 2015, 31 (09) : 407 - 412
  • [10] Peroxiredoxins in Parasites
    Gretes, Michael C.
    Poole, Leslie B.
    Karplus, P. Andrew
    [J]. ANTIOXIDANTS & REDOX SIGNALING, 2012, 17 (04) : 608 - 633
1234