Vimentin binds IRAP and is involved in GLUT4 vesicle trafficking

被引：21

作者：

Hirata, Yohko ^{[2
]}

Hosaka, Toshio ^{[1
]}

Iwata, Takeo ^{[3
]}

Le, Chung T. K. ^{[2
]}

Jambaldorj, Bayasgalan ^{[2
]}

Teshigawara, Kiyoshi ^{[2
]}

Harada, Nagakatsu ^{[2
]}

Sakaue, Hiroshi ^{[2
]}

Sakai, Tohru ^{[1
]}

Yoshimoto, Katsuhiko ^{[3
]}

Nakaya, Yutaka ^{[2
]}

机构：

[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Publ Hlth & Appl Nutr, Tokushima 7708503, Japan

[2] Univ Tokushima, Dept Nutr & Metab, Inst Hlth Biosci, Tokushima 7708503, Japan

[3] Univ Tokushima, Dept Med Pharmacol, Inst Hlth Biosci, Tokushima 7708503, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 405卷 / 01期

关键词：

Vimentin; GLUT4; IRAP; INSULIN-REGULATED AMINOPEPTIDASE; STIMULATED GLUCOSE-UPTAKE; RAT ADIPOSE-CELLS; 3T3-L1; ADIPOCYTES; GLUT4-CONTAINING VESICLES; MEMBRANE AMINOPEPTIDASE; CYTOSKELETAL INTEGRITY; STORAGE-VESICLES; MAJOR PROTEIN; TRANSLOCATION;

D O I：

10.1016/j.bbrc.2010.12.134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin-responsive aminopeptidase (1RAP) and GLUT4 are two major cargo proteins of GLUT4 storage vesicles (GSVs) that are translocated from a postendosomal storage compartment to the plasma membrane (PM) in response to insulin. The cytoplasmic region of IRAP is reportedly involved in retention of GSVs. In this study, vimentin was identified using the cytoplasmic domain of IRAP as bait. The validity of this interaction was confirmed by pull-down assays and immunoprecipitation in 3T3-L1 adipocytes. In addition, it was shown that GLUT4 translocation to the PM by insulin was decreased in vimentin-depleted adipocytes, presumably due to dispersing GSVs away from the cytoskeleton. These findings suggest that the IRAP binding protein, vimentin, plays an important role in retention of GSVs. (c) 2011 Elsevier Inc. All rights reserved.

引用

页码：96 / 101

页数：6

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