Label-free optical imaging of mitochondria in live cells

被引：66
作者：
Lasne, David
Blab, Gerhard. A.
De Giorgi, Francesca
Ichas, Francois
Lounis, Brahim
Cognet, Laurent [1 ]
机构：
[1] Univ Bordeaux 1, Ctr Phys Mol Opt & Hertzienne, CNRS, F-33405 Talence, France
[2] INSERM, Inst Bergonie, F-33000 Bordeaux, France
来源：
OPTICS EXPRESS | 2007年 / 15卷 / 21期
关键词：
D O I：
10.1364/OE.15.014184
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The far-field optical imaging of mitochondria of live cells without the use of any label is demonstrated. It uses a highly sensitive photothermal method and has a resolution comparable to confocal fluorescence setups. The morphological states of mitochondria were followed under different physiological treatments, and the role of cytochrome c was ruled out as the main origin of the photothermal signals. This label free optical method provides a high contrast imaging of live mitochondria and should find many applications in biosciences. (c) 2007 Optical Society of America.
引用
收藏
页码：14184 / 14193
页数：10
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