Monitoring platelet dependent thrombin generation in mice

被引：22

作者：

Dargaud, Yesim ^{[1
,2
,3
]}

Spronk, Henri M. H. ^{[2
,3
]}

Leenders, Peter ^{[2
,3
]}

Hemker, H. Coenraad ^{[4
]}

Ten Cate, Hugo ^{[2
,3
]}

机构：

[1] Univ Lyon 1, Unite Hemostase Clin, Hop Edouard Herriot, F-69003 Lyon, France

[2] Maastricht Univ, Lab Clin Thrombosis & Haemostasis, Dept Internal Med, Maastricht, Netherlands

[3] Maastricht Univ, Cardiovasc Res Inst Maastricht, Maastricht, Netherlands

[4] Synapse Bv, Cardiovasc Res Inst Maastricht, Maastricht, Netherlands

来源：

THROMBOSIS RESEARCH | 2010年 / 126卷 / 05期

关键词：

Thrombin generation; Mouse; Platelet; Corn trypsin inhibitor; Activated protein C resistance; CALIBRATED AUTOMATED THROMBOGRAPHY; MOUSE PLASMA; COAGULATION;

D O I：

10.1016/j.thromres.2010.08.007

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Calibrated automated thrombin generation assay was adapted to measure thrombin generation in platelet rich plasma from mice. Vena cava phlebotomy appeared the best technique for blood sampling. The concentration-effect curves of tissue factor and platelet count have been determined. Corn trypsin inhibitor 2 mu M inhibits contact activation effectively. Recombinant human thrombomodulin does not inhibit thrombin generation in mouse plasma but activated protein C (20 nM) does. Thrombin generation was dose dependently diminished by low molecular weight heparin and increased by high concentrations of exogenous factor VIII i.e. the assay can detect both hypo-and hypercoagulability. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：436 / 441

页数：6

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