The mutagenic activity of the S-nitrosoglutathione/glutathione system in Salmonella typhimurium TA1535

被引：4

作者：

Abu-Shakra, A ^{[1
]}

机构：

[1] N Carolina Cent Univ, Dept Biol, Durham, NC 27707 USA

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2003年 / 539卷 / 1-2期

关键词：

mutagenesis; nitric oxide; Ames strains; oxidative damage; S-nitrosothiols;

D O I：

10.1016/S1383-5718(03)00131-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

S-nitrosoglutathine (GSNO) and reduced glutathione (GSH) were tested for mutagenicity against strain Salmonella typhimurium TA1535 in the Ames Standard plate incorporation assay. Neither GSNO not GSH were mutagenic when tested alone. In combination, the GSNO/GSH system induced a positive mutagenic response that ranged from 3 to 20x over background at concentrations of 10 to 50 mumol (micromol)per plate, respectively. This mutagenic response can be attributable to the generation nitric oxide, among the many other reactive products generated by the reaction of GSNO with GSH. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：203 / 206

页数：4

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