Production of Ligninolytic Enzymes by Cultures of White Rot Fungi

被引：0

作者：

Gorska, Ewa B. ^{[1
]}

Jankiewicz, Urszula ^{[2
]}

Dobrzynski, Jakub ^{[1
]}

Galazka, Agnieszka ^{[2
]}

Sitarek, Monika ^{[3
]}

Gozdowski, Dariusz ^{[5
]}

Russel, Stefan ^{[4
]}

Kowalczyk, Pawel ^{[6
]}

机构：

[1] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Microbiol Biol, Warsaw, Poland

[2] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Biochem, Warsaw, Poland

[3] Warsaw Univ Life Sci SGGW, Dept Vegetable & Med Plants, Fac Hort Biotechnol & Landscape Architecture, Warsaw, Poland

[4] Inst Technol & Life Sci Falenty, Raszyn, Poland

[5] Warsaw Univ Life Sci SGGW, Fac Agr & Biol, Dept Expt Stat & Bioinformat, Warsaw, Poland

[6] Bionicum LTD, Warsaw, Poland

来源：

POLISH JOURNAL OF MICROBIOLOGY | 2014年 / 63卷 / 04期

关键词：

Basidiomycota; degradation of straw; laccase; peroxidase; PLEUROTUS-OSTREATUS; LACCASE; DEGRADATION; PEROXIDASE;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Some Basidiomycota were chosen for studies of key ligninases synthesis (25 degrees C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzyme isoforms (ca 30 and 16 kDa). In T. versicolor culture active laccase protein with highest molecular mass ca 65 kDa was found. P. sajor-caju yielded three different peroxidase isoforms. Ligninase biosynthesis depended on strain, straw fragmentation extent, culture method and growth medium.

引用

页码：461 / 465

页数：5

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