Diode laser based detection of single molecules in solutions

被引：33

作者：

Sauer, M ^{[1
]}

Drexhage, KH ^{[1
]}

Zander, C ^{[1
]}

Wolfrum, J ^{[1
]}

机构：

[1] UNIV GESAMTHSCH SIEGEN,INST PHYS CHEM,D-57068 SIEGEN,GERMANY

来源：

CHEMICAL PHYSICS LETTERS | 1996年 / 254卷 / 3-4期

关键词：

D O I：

10.1016/0009-2614(96)00303-X

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Using a confocal microscope we studied bursts of fluorescence photons from single dye molecules that were excited at 632 nm with a CW diode laser or helium neon laser. The measurements were performed with new rhodamine derivatives,in ethylene glycol solution. The potential of this method for future construction of a low-cost instrument for single-molecule detection is pointed out. To demonstrate the utility of our new dyes in bioanalytical applications we showed the single-molecule detection of labeled oligonucleotides in water.

引用

页码：223 / 228

页数：6

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