Diode laser based detection of single molecules in solutions

被引：33
作者：
Sauer, M [1 ]
Drexhage, KH [1 ]
Zander, C [1 ]
Wolfrum, J [1 ]
机构：
[1] UNIV GESAMTHSCH SIEGEN,INST PHYS CHEM,D-57068 SIEGEN,GERMANY
来源：
CHEMICAL PHYSICS LETTERS | 1996年 / 254卷 / 3-4期
关键词：
D O I：
10.1016/0009-2614(96)00303-X
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Using a confocal microscope we studied bursts of fluorescence photons from single dye molecules that were excited at 632 nm with a CW diode laser or helium neon laser. The measurements were performed with new rhodamine derivatives,in ethylene glycol solution. The potential of this method for future construction of a low-cost instrument for single-molecule detection is pointed out. To demonstrate the utility of our new dyes in bioanalytical applications we showed the single-molecule detection of labeled oligonucleotides in water.
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页码：223 / 228
页数：6
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