CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection

被引：0

作者：

Garcia-Elorriaga, Guadalupe ^{[1
]}

Esparza-Garcia, Amalia ^{[2
]}

Mendez-Rojas, Claudia ^{[1
]}

del Rey-Pineda, Guillermo

Gonzalez-Bonilla, Cesar ^{[1
]}

机构：

[1] Hosp Infectol Dr Federico Mendez, Ctr Med Nacl La Raza, Unidad Invest Inmunol & Infectol, IMSS, Mexico City 02990, DF, Mexico

[2] Hosp Infectol Dr Federico Mendez, Ctr Med Nacl La Raza, Serv Infectol Pediat, IMSS, Mexico City 02990, DF, Mexico

来源：

REVISTA DE INVESTIGACION CLINICA-CLINICAL AND TRANSLATIONAL INVESTIGATION | 2012年 / 64卷 / 01期

关键词：

Enterovirus; RT-PCR; Infection of the CNS; CEREBROSPINAL-FLUID SPECIMENS; POLYMERASE-CHAIN-REACTION; ASEPTIC-MENINGITIS; MOLECULAR DIAGNOSIS; CLINICAL-SAMPLES; ENCEPHALITIS; CHILDREN; MANAGEMENT; REGION; ASSAY;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. We standardized the RT-PCR pan viral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years. Material and methods. RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system. Results. 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments. Conclusions. Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.

引用

页码：59 / 66

页数：8

共 33 条

[1] Impact of Rapid Enterovirus Molecular Diagnosis on the Management of Infants, Children, and Adults with Aseptic Meningitis [J].

Archimbaud, C. ;

Chambon, M. ;

Bailly, J. L. ;

Petit, I. ;

Henquell, C. ;

Mirand, A. ;

Aublet-Cuvelier, B. ;

Ughetto, S. ;

Beytout, J. ;

Clavelou, P. ;

Labbe, A. ;

Philippe, P. ;

Schmidt, J. ;

Regagnon, C. ;

Traore, O. ;

Peigue-Lafeuille, H. .

JOURNAL OF MEDICAL VIROLOGY, 2009, 81 (01) :42-48

[2] Complete genomic Sequencing shows that Polioviruses and members of human enterovirus species C are closely related in the noncapsid coding region [J].

Brown, B ;

Oberste, MS ;

Maher, K ;

Pallansch, MA .

JOURNAL OF VIROLOGY, 2003, 77 (16) :8973-8984

[3] Viral etiologies of encephalitis in Thai children [J].

Chokephaibulkit, K ;

Kankirawatana, P ;

Apintanapong, S ;

Pongthapisit, V ;

Yoksan, S ;

Kositanont, U ;

Puthavathana, P .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2001, 20 (02) :216-218

[4]

Chomczynski P, 1998, DESIGNING CLIN RES E, P325

[5]

Coria-Lorenzo JJ, 2001, REV MEX PED, V68, P252

[6] Molecular diagnosis of CNS viral infections [J].

Davis, LE ;

Tyler, KL .

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 2005, 76 (01) :10-10

[7] Childhood encephalitis in Sweden: Etiology, clinical presentation and outcome [J].

Fowler, A. ;

Stodberg, T. ;

Eriksson, M. ;

Wickstrom, R. .

EUROPEAN JOURNAL OF PAEDIATRIC NEUROLOGY, 2008, 12 (06) :484-490

[8]

Fu Jin, 2007, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi, V21, P316

[9] Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology [J].

Hong, JiYoung ;

Kang, Byunghak ;

Kim, Ahyoun ;

Hwang, Seoyoun ;

Lee, Sunwha ;

Kim, Jonghyen ;

Lee, Hyun-Young ;

Kang, Sang-Hyeon ;

Cheon, Doo-Sung .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (02) :615-616

[10] COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOME OF COXSACKIEVIRUS-B1 [J].

IIZUKA, N ;

KUGE, S ;

NOMOTO, A .

VIROLOGY, 1987, 156 (01) :64-73

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