Identification of novel phosphatidic acid-binding proteins in the rat brain

被引：10

作者：

Park, Chihu ^{[1
,2
]}

Kang, Du-Seock ^{[3
]}

Shin, Geon-Hoon ^{[2
]}

Seo, Jeongkon ^{[3
,4
]}

Kim, Hyein ^{[2
,5
]}

Suh, Pann-Ghill ^{[3
]}

Bae, Chang-Dae ^{[1
]}

Shin, Joo-Ho ^{[2
,5
]}

机构：

[1] Sungkyunkwan Univ, Sch Med, Dept Mol Cell Biol, Suwon 440746, Gyeonggi Do, South Korea

[2] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Mass Spectrometry,Res Core Facil, Suwon 440746, Gyeonggi Do, South Korea

[3] Ulsan Natl Inst Sci & Technol, Sch Life Sci, Ulsan, South Korea

[4] Ulsan Natl Inst Sci & Technol, UNIST Cent Res Facil, Ulsan, South Korea

[5] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Div Pharmacol,Dept Mol Cell Biol, Suwon 440746, Gyeonggi Do, South Korea

来源：

NEUROSCIENCE LETTERS | 2015年 / 595卷

关键词：

Phosphatidic acid; Lipid-protein interaction; LC-MS/MS; Brain;

D O I：

10.1016/j.neulet.2015.04.012

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Phosphatidic acid (PA) is an abundant negatively-charged phospholipid and has long been considered to be an important signaling molecule in diverse cellular events. Thus, the identification of proteins that specifically interact with PA is of considerable interest to understand the regulatory roles of PA. Herein, lipid-affinity purification and mass spectrometric analysis reveals 43 proteins, 19 known and 24 novel, as PA-binding proteins. A lipid-protein overlay assay confirmed that GDI1, PACSIN1, and DPYSL2 interact with not only with PA but also with other phospholipids. These results might be helpful for deciphering the functional effect of PA in the brain. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

引用

页码：108 / 113

页数：6

共 17 条

[1] The Coffin-Lowry Syndrome-Associated Protein RSK2 Regulates Neurite Outgrowth through Phosphorylation of Phospholipase D1 (PLD1) and Synthesis of Phosphatidic Acid [J].

Ammar, Mohamed-Raafet ;

Humeau, Yann ;

Hanauer, Andre ;

Nieswandt, Bernard ;

Bader, Marie-France ;

Vitale, Nicolas .

JOURNAL OF NEUROSCIENCE, 2013, 33 (50) :19470-19479

[2] Phosphatidic acid, a key intermediate in lipid metabolism [J].

Athenstaedt, K ;

Daum, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 266 (01) :1-16

[3] Software Tool for Researching Annotations of Proteins: Open-Source Protein Annotation Software with Data Visualization [J].

Bhatia, Vivek N. ;

Perlman, David H. ;

Costello, Catherine E. ;

McComb, Mark E. .

ANALYTICAL CHEMISTRY, 2009, 81 (23) :9819-9823

[4] Phosphatidic acid-mediated mitogenic activation of mTOR signaling [J].

Fang, YM ;

Vilella-Bach, M ;

Bachmann, R ;

Flanigan, A ;

Chen, J .

SCIENCE, 2001, 294 (5548) :1942-1945

[5] The cAMP-specific phosphodiesterase PDE4D3 is regulated by phosphatidic acid binding -: Consequences for cAMP signaling pathway and characterization of a phosphatidic acid binding site [J].

Grange, M ;

Sette, C ;

Cuomo, M ;

Conti, M ;

Lagarde, M ;

Prigent, AF ;

Némoz, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (43) :33379-33387

[6] STRING 8-a global view on proteins and their functional interactions in 630 organisms [J].

Jensen, Lars J. ;

Kuhn, Michael ;

Stark, Manuel ;

Chaffron, Samuel ;

Creevey, Chris ;

Muller, Jean ;

Doerks, Tobias ;

Julien, Philippe ;

Roth, Alexander ;

Simonovic, Milan ;

Bork, Peer ;

von Mering, Christian .

NUCLEIC ACIDS RESEARCH, 2009, 37 :D412-D416

[7] Tight binding inhibition of protein phosphatase-1 by phosphatidic acid - Specificity of inhibition by the phospholipid [J].

Jones, JA ;

Hannun, YA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (18) :15530-15538

[8] Phospholipase D signalling and its involvement in neurite outgrowth [J].

Kanaho, Yasunori ;

Funakoshi, Yuji ;

Hasegawa, Hiroshi .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2009, 1791 (09) :898-904

[9] The biguanide metformin alters phosphoproteomic profiling in mouse brain [J].

Khang, Rin ;

Park, ChiHu ;

Shin, Joo-Ho .

NEUROSCIENCE LETTERS, 2014, 579 :145-150

[10] Disruption of diacylglycerol kinase delta (DGKD) associated with seizures in humans and mice [J].

Leach, Natalia T. ;

Sun, Yi ;

Michaud, Sebastien ;

Zheng, Yi ;

Ligon, Keith L. ;

Ligon, Azra H. ;

Sander, Thomas ;

Korf, Bruce R. ;

Lu, Weining ;

Harris, David J. ;

Gusella, James F. ;

Maas, Richard L. ;

Quade, Bradley J. ;

Cole, Andrew J. ;

Kelz, Max B. ;

Morton, Cynthia C. .

AMERICAN JOURNAL OF HUMAN GENETICS, 2007, 80 (04) :792-799

← 1 2 →