Polymerase chain reaction restriction fragment length polymorphism analysis of a short fragment of the cytochrome b gene for identification of flatfish species

被引：24

作者：

Céspedes, A ^{[1
]}

García, T ^{[1
]}

Carrera, E ^{[1
]}

González, I ^{[1
]}

Sanz, B ^{[1
]}

Hernández, PE ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol Higiene & Tecnol Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF FOOD PROTECTION | 1998年 / 61卷 / 12期

关键词：

D O I：

10.4315/0362-028X-61.12.1684

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). PCR amplification of the cytochrome b gene using a universal primer together with a primer specifically designed as a part of this study produced a 201-bp fragment in all species analyzed. Digestions of the PCR products with Sau3Al, BsmAl, Rsa1, and Mn/l endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.

引用

页码：1684 / 1685

页数：2

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