The Protective Effect of ENA Actimineral Resource A on CCl4-Induced Liver Injury in Rats

被引：7

作者：

Hong, Il-Hwa ^{[1
]}

Ji, Hoon ^{[1
]}

Hwa, Sung-Yong ^{[2
]}

Jeong, Won-Il ^{[1
]}

Jeong, Da-Hee ^{[1
]}

Do, Sun-Hee ^{[3
]}

Kim, Ji-Min ^{[4
]}

Ki, Mi-Ran ^{[1
]}

Park, Jin-Kyu ^{[1
]}

Goo, Moon-Jung ^{[1
]}

Hwang, Ok-Kyung ^{[1
]}

Hong, Kyung-Sook ^{[1
]}

Han, Jung-Youn ^{[1
]}

Chung, Hae-Young ^{[4
]}

Jeong, Kyu-Shik ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Pathol, Coll Vet Med, Taegu 702701, South Korea

[2] Jinju Bio Food, Jinju 660931, South Korea

[3] Konkuk Univ, Dept Clin Pathol, Coll Vet Med, Seoul 143701, South Korea

[4] Pusan Natl Univ, Longev Life Sci & Technol Inst, Dept Pharm, Pusan 609735, South Korea

来源：

MARINE BIOTECHNOLOGY | 2011年 / 13卷 / 03期

关键词：

ENA actimineral resource A; Antioxidant capability; Hepatoxicity; Seaweed; Carbon tetrachloride; CARBON-TETRACHLORIDE; IN-VIVO; LIPID-PEROXIDATION; OXIDATIVE STRESS; GENE-EXPRESSION; ENZYMES; METABOLISM; KININOGEN; PHASE; NRF2;

D O I：

10.1007/s10126-010-9317-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

ENA Actimineral Resource A (ENA-A) is alkaline water that is composed of refined edible cuttlefish bone and two different species of seaweed, Phymatolithon calcareum and Lithothamnion corallioides. In the present study, ENA-A was investigated as an antioxidant to protect against CCl4-induced oxidative stress and hepatotoxicity in rats. Liver injury was induced by either subacute or chronic CCl4 administration, and the rats had free access to tap water mixed with 0% (control group) or 10% (v/v) ENA-A for 5 or 8 weeks. The results of histological examination and measurement of antioxidant activity showed that the reactive oxygen species production, lipid peroxidation, induction of CYP2E1 were decreased and the antioxidant activity, including glutathione and catalase production, was increased in the ENA-A groups as compared with the control group. On 2-DE gel analysis of the proteomes, 13 differentially expressed proteins were obtained in the ENA-A groups as compared with the control group. Antioxidant proteins, including glutathione S-transferase, kelch-like ECH-associated protein 1, and peroxiredoxin 1, were increased with hepatocyte nuclear factor 3-beta and serum albumin precursor, and kininogen precursor decreased more in the ENA-A groups than compared to the control group. In conclusion, our results suggest that ENA-A does indeed have some protective capabilities against CCl4-induced liver injury through its antioxidant function.

引用

页码：462 / 473

页数：12

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