Assessing the reliability of microscopy and rapid diagnostic tests in malaria diagnosis in areas with varying parasite density among older children and adult patients in Nigeria

被引:17
作者
Ayogu, E. E. [1 ,2 ]
Ukwe, C., V [1 ]
Nna, E. O. [3 ]
机构
[1] Univ Nigeria, Fac Pharmaceut Sci, Dept Clin Pharm & Pharm Management, Nsukka, Nigeria
[2] Minist Hlth, Dist Hosp Nsukka, Pharm Unit, Nsukka, Nigeria
[3] Univ Nigeria, Fac Hlth Sci & Technol, Safety Mol Pathol Lab, Enugu Campus, Nsukka, Enugu State, Nigeria
关键词
Diagnosis; malaria; malaria diagnosis; microscopy; polymerase chain reaction (PCR); rapid diagnostic test (RDT); PLASMODIUM-FALCIPARUM MALARIA; PCR; CHALLENGES; QUALITY; FIELD;
D O I
10.4103/0022-3859.183167
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Current malaria control strategies are based on early diagnosis and appropriate treatment of malaria cases. The study aimed at comparing the performance of blood film microscopy and rapid diagnostic test (RDT) in Plasmodium falciparum detection in patients >= 6 years of age. Materials and Methods: A total of 154 consecutive pyretic patients aged 6-62 years were enrolled, sampled, and tested for malaria using RDT (first response) and microscopy by Giemsa staining. Genomic DNA was extracted after saponin hemolysis and nested polymerase chain reaction (PCR) was used to detect Plasmodium falciparum. The endpoints were sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Results: Of the 154 patients, 80 (51.9%) had fever of >= 37.5 degrees C. 106 (68.8%) were positive by First response(R), 132 (85.7%) by microscopy, and 121 (78.6%) by PCR. The sensitivity, specificity, PPV, and NPV of first response compared to microscopic method were 82.2%, 100.0%, 100.0%, and 34.3%, respectively, while it was 75.4%, 75.0%, 95.3%, and 31.2%, respectively, when compared to PCR. The sensitivity, specificity, PPV, and NPV of the microscopic method compared to PCR were 92.3%, 50.0%, 90.91%, and 54.5%, respectively. There was a significant difference in the performance of RDT and film microscopy methods (P <= 0.05). Conclusion: Microscopy performed better and is more reliable than first response (RDT) in areas with low parasite density among patients >= 6 years of age. Rapid diagnostic tests could be useful in aareas with high parasite density as an alternative to smear microscopy
引用
收藏
页码:150 / 156
页数:7
相关论文
共 32 条
[1]   Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries [J].
Abba, Katharine ;
Deeks, Jonathan J. ;
Olliaro, Piero ;
Naing, Cho-Min ;
Jackson, Sally M. ;
Takwoingi, Yemisi ;
Donegan, Sarah ;
Garner, Paul .
COCHRANE DATABASE OF SYSTEMATIC REVIEWS, 2011, (07)
[2]   Estimating malaria parasite density: assumed white blood cell count of 10,000/μl of blood is appropriate measure in Central Ghana [J].
Adu-Gyasi, Dennis ;
Adams, Mohammed ;
Amoako, Sabastina ;
Mahama, Emmanuel ;
Nsoh, Maxwell ;
Amenga-Etego, Seeba ;
Baiden, Frank ;
Asante, Kwaku Poku ;
Newton, Sam ;
Owusu-Agyei, Seth .
MALARIA JOURNAL, 2012, 11
[3]   Towards a precise test for malaria diagnosis in the Brazilian Amazon: comparison among field microscopy, a rapid diagnostic test, nested PCR, and a computational expert system based on artificial neural networks [J].
Andrade, Bruno B. ;
Reis-Filho, Antonio ;
Barros, Austeclino M. ;
Souza-Neto, Sebastiao M. ;
Nogueira, Lucas L. ;
Fukutani, Kiyoshi F. ;
Camargo, Erney P. ;
Camargo, Luis M. A. ;
Barral, Aldina ;
Duarte, Angelo ;
Barral-Netto, Manoel .
MALARIA JOURNAL, 2010, 9
[4]   Evaluation of three parasite lactate dehydrogenase-based rapid diagnostic tests for the diagnosis of falciparum and vivax malaria [J].
Ashley, Elizabeth A. ;
Touabi, Malek ;
Ahrer, Margareta ;
Hutagalung, Robert ;
Htun, Khayae ;
Luchavez, Jennifer ;
Dureza, Christine ;
Proux, Stephane ;
Leimanis, Mara ;
Lwin, Myo Min ;
Koscalova, Alena ;
Comte, Eric ;
Hamade, Prudence ;
Page, Anne-Laure ;
Nosten, Francois ;
Guerin, Philippe J. .
MALARIA JOURNAL, 2009, 8
[5]   Are rapid diagnostic tests more accurate in diagnosis of plasmodium falciparum malaria compared to microscopy at rural health centres? [J].
Batwala, Vincent ;
Magnussen, Pascal ;
Nuwaha, Fred .
MALARIA JOURNAL, 2010, 9
[6]   Ensuring quality and access for malaria diagnosis: how can it be achieved? [J].
Bell, David ;
Wongsrichanalai, Chansuda ;
Barnwell, John W. .
NATURE REVIEWS MICROBIOLOGY, 2006, 4 (09) :682-695
[7]   False-positive results of a Plasmodium falciparum histidine-rich protein 2-detecting malaria rapid diagnostic test due to high sensitivity in a community with fluctuating low parasite density [J].
Bell, DR ;
Wilson, DW ;
Martin, LB .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2005, 73 (01) :199-203
[8]   The heat stability of Plasmodium lactate dehydrogenase-based and histidine-rich protein 2-based malaria rapid diagnostic tests [J].
Chiodini, Peter L. ;
Bowers, Katherine ;
Jorgensen, Pernille ;
Barnwell, John W. ;
Grady, Katharine K. ;
Luchavez, Jenny ;
Moody, Anthony H. ;
Cenizal, Audie ;
Bell, David .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2007, 101 (04) :331-337
[9]   Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand [J].
Coleman, Russell E. ;
Sattabongkot, Jetsumon ;
Promstaporm, Sommai ;
Maneechai, Nongnuj ;
Tippayachai, Bousaraporn ;
Kengluecha, Ampornpan ;
Rachapaew, Nattawan ;
Zollner, Gabriela ;
Miller, Robert Scott ;
Vaughan, Jefferson A. ;
Thimasarn, Krongtong ;
Khuntirat, Benjawan .
MALARIA JOURNAL, 2006, 5 (1)
[10]  
Federal Ministry of Health, 2004, MAL CONTR NIG STRAT, P58