Molecular profiling of bacterial species in the rabbit caecum

被引:49
作者
Abecia, L
Fondevila, M
Balcells, J
Edwards, JE
Newbold, CJ
McEwan, NR [1 ]
机构
[1] Rowett Res Inst, Aberdeen AB21 9SB, Scotland
[2] Univ Zaragoza, Dept Prod Anim & Ciencia Alimentos, Zaragoza, Spain
[3] Inst Food Res, Inst Grassland & Environm Res, Welsh Plant Breeding Stn, Aberystwyth SY23 3EB, Dyfed, Wales
[4] Univ Coll Wales, Inst Rural Studies, Aberystwyth SY23 3AL, Dyfed, Wales
关键词
rabbit; caecum; bacterial diversity; phylogenetic analysis;
D O I
10.1016/j.femsle.2005.01.028
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The diversity of bacteria present in the caecum of the rabbit was investigated. Partial bacterial 16S rRNA genes from a digested sample collected from the caecum of an adult rabbit were amplified by PCR. Sequence analysis of the amplified fragments indicated highest similarity was to bacterial sequences previously described from other gut environments. However, only one sequence showed significant identity (97% threshold) to any previously described bacterial 16S rRNA genes. Furthermore, most of the sequences clustered together in groups lacking representatives from sequences already described, suggesting that the rabbit caecal flora contains organisms not previously described. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:111 / 115
页数:5
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