1H-NMR-based metabolomics of tumor tissue for the metabolic characterization of rat hepatocellular carcinoma formation and metastasis

被引:37
作者
Wang, Juan [1 ]
Zhang, Shu [1 ]
Li, Zongfang [1 ]
Yang, Jun [2 ]
Huang, Chen [3 ]
Liang, Rongrui [1 ]
Liu, Zhongwei [1 ]
Zhou, Rui [1 ]
机构
[1] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Gen Surg, Sch Med, Xian 710004, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Pathol, Sch Med, Xian 710004, Shaanxi, Peoples R China
[3] Xi An Jiao Tong Univ, Sch Med, Key Lab Environm & Genes Related Dis, Educ Minist, Xian 710004, Shaanxi, Peoples R China
关键词
Metabolomics; NMR; HCC; Metastasis; POSITRON-EMISSION-TOMOGRAPHY; CANCER; METABONOMICS; CELLS; EXPRESSION; SPECTROSCOPY; PROFILES;
D O I
10.1007/s13277-010-0116-7
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The high mortality figures for hepatocellular carcinoma (HCC) are mostly due to high recurrence and metastasis rates. However, the metabolic characteristics of HCC metastasis have not been studied extensively. In this study, we attempted to elucidate the metabolite profile of HCC formation and metastasis through proton nuclear magnetic resonance (H-1-NMR)-based metabolomics. We first established a hepatocellular carcinoma with lung metastasis (HLM) rat model by exposure to diethylnitrosamine. Fifteen rats were then divided into three groups based on pathologic changes: HCC, HLM, and controls. The metabolite profiles of extracts from tumor tissue were obtained using high-resolution H-1-NMR. One-way ANOVA was used to compare the metabolite levels among the three groups. Multivariate statistical analysis (specifically, unsupervised principal components analysis and supervised partial least squares discriminant analysis (PLS-DA)) were used for HCC and HLM metabolite profiling and data interpretation. PLS-DA models could discern HCC or HLM rats from normal (control) rats. Tumor tissue from HLM showed changes in glucose, lactate, choline, lipids, and some amino acids such as glycine. The results of this pilot study suggest that alterations in glycolysis and the metabolism of glycine and choline occur during HCC invasion and metastasis.
引用
收藏
页码:223 / 231
页数:9
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