Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins

被引：318

作者：

Kawano, Fuun ^{[1
]}

Suzuki, Hideyuki ^{[1
]}

Furuya, Akihiro ^{[1
]}

Sato, Moritoshi ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Meguro Ku, Tokyo 1538902, Japan

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

日本学术振兴会;

关键词：

SPATIOTEMPORAL CONTROL; MEDIATED INDUCTION; GENE-EXPRESSION; LIVING CELLS; LIGHT; ACTIVATION; SYSTEM; DYNAMICS; MOTILITY; DOMAIN;

D O I：

10.1038/ncomms7256

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Optogenetic methods take advantage of photoswitches to control the activity of cellular proteins. Here, we completed a multi-directional engineering of the fungal photoreceptor Vivid to develop pairs of distinct photoswitches named Magnets. These new photoswitches were engineered to recognize each other based on the electrostatic interactions, thus preventing homodimerization and enhancing light-induced heterodimerization. Furthermore, we tuned the switch-off kinetics by four orders of magnitude and developed several variants, including those with substantially faster kinetics than any of the other conventional dimerization-based blue spectrum photoswitches. We demonstrate the utility of Magnets as powerful tools that can optogenetically manipulate molecular processes in biological systems.

引用

页数：8

共 32 条

[11] Spatiotemporal control of cell signalling using a light-switchable protein interaction [J].

Levskaya, Anselm ;

Weiner, Orion D. ;

Lim, Wendell A. ;

Voigt, Christopher A. .

NATURE, 2009, 461 (7266) :997-1001

[12] Designing Photoswitchable Peptides Using the AsLOV2 Domain [J].

Lungu, Oana I. ;

Hallett, Ryan A. ;

Choi, Eun Jung ;

Aiken, Mary J. ;

Hahn, Klaus M. ;

Kuhlman, Brian .

CHEMISTRY & BIOLOGY, 2012, 19 (04) :507-517

[13]

Motta-Mena LB, 2014, NAT CHEM BIOL, V10, P196, DOI [10.1038/NCHEMBIO.1430, 10.1038/nchembio.1430]

[14] Optogenetic tools for mammalian systems [J].

Mueller, Konrad ;

Weber, Wilfried .

MOLECULAR BIOSYSTEMS, 2013, 9 (04) :596-608

[15] Combinatorial library screening for developing an improved split-firefly luciferase fragment-assisted complementation system for studying protein-protein interactions [J].

Paulmurugan, Ramasamy ;

Gambhir, Sanjiv S. .

ANALYTICAL CHEMISTRY, 2007, 79 (06) :2346-2353

[16] Lifeact: a versatile marker to visualize F-actin [J].

Riedl, Julia ;

Crevenna, Alvaro H. ;

Kessenbrock, Kai ;

Yu, Jerry Haochen ;

Neukirchen, Dorothee ;

Bista, Michal ;

Bradke, Frank ;

Jenne, Dieter ;

Holak, Tad A. ;

Werb, Zena ;

Sixt, Michael ;

Wedlich-Soldner, Roland .

NATURE METHODS, 2008, 5 (07) :605-607

[17] Production of PtdInsP3 at endomembranes is triggered by receptor endocytosis [J].

Sato, M ;

Ueda, Y ;

Takagi, T ;

Umezawa, Y .

NATURE CELL BIOLOGY, 2003, 5 (11) :1016-1022

[18] Imaging diacylglycerol dynamics at organelle membranes [J].

Sato, Moritoshi ;

Ueda, Yoshibumi ;

Umezawa, Yoshio .

NATURE METHODS, 2006, 3 (10) :797-799

[19]

Sawano A, 2000, Nucleic Acids Res, V28, pE78, DOI 10.1093/nar/28.16.e78

[20] A light-switchable gene promoter system [J].

Shimizu-Sato, S ;

Huq, E ;

Tepperman, JM ;

Quail, PH .

NATURE BIOTECHNOLOGY, 2002, 20 (10) :1041-1044

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