Regulation of coactivator complex assembly and function by protein arginine methylation and demethylimination

被引:183
作者
Lee, YH
Coonrod, SA
Kraus, WL
Jelinek, MA
Stallcup, MR
机构
[1] Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA
[2] Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
[3] Cornell Univ, Weill Med Coll, Dept Med Genet, New York, NY 10021 USA
[4] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
[5] Upstate Biotechnol, Lake Placid, NY 12946 USA
关键词
transcriptional regulation;
D O I
10.1073/pnas.0407159102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nuclear receptors activate transcription by recruiting multiple coactivators to the promoters of specific target genes. The functional synergy of the p160 coactivators [steroid receptor coactivator-1, glucocorticoid receptor interacting protein (GRIP1), or the activator for thyroid hormone and retinoid receptors], the histone acetyltransferases cAMP response element binding protein binding protein (CBP) and p300 and the histone methyltransferase coactivator-associated arginine methyltransferase (CARM1) depends on the methyltransferase activity of CARM1. CARM1 methylates histone H3 and other factors including the N-terminal region of p300. Here, we report that CARM1 also methylates Arg-2142 within the C-terminal GRIP1 binding domain (GBD) of p300. In the GBD, both Arg-2088 and Arg-2142 are important for binding GRIP1. Methylation of Arg-2142 inhibits the bimolecular interaction of GRIN to p300 in vitro and in vivo. This methylation mark of p300 GBD is removed by peptidyl deiminase 4, thereby enhancing the p300-GRIP1 interaction. These methylation and demethylimination events also alter the conformation and activity of the coactivator complex and regulate estrogen receptor-mediated transcription, and they thus represent unique mechanisms for regulating coactivator complex assembly, conformation, and function.
引用
收藏
页码:3611 / 3616
页数:6
相关论文
共 34 条
[1]   Ordered cooperative functions of PRMT1, p300, and CARM1 in transcriptional activation by p53 [J].
An, W ;
Kim, J ;
Roeder, RG .
CELL, 2004, 117 (06) :735-748
[2]   Histone methylation: Dynamic or static? [J].
Bannister, AJ ;
Schneider, R ;
Kouzarides, T .
CELL, 2002, 109 (07) :801-806
[3]   Dynamic behavior of transcription factors on a natural promoter in living cells [J].
Becker, M ;
Baumann, C ;
John, S ;
Walker, DA ;
Vigneron, M ;
McNally, JG ;
Hager, GL .
EMBO REPORTS, 2002, 3 (12) :1188-1194
[4]   Regulation of transcription by a protein methyltransferase [J].
Chen, DG ;
Ma, H ;
Hong, H ;
Koh, SS ;
Huang, SM ;
Schurter, BT ;
Aswad, DW ;
Stallcup, MR .
SCIENCE, 1999, 284 (5423) :2174-2177
[5]   Synergistic, p160 coactivator-dependent enhancement of estrogen receptor function by CARM1 and p300 [J].
Chen, DG ;
Huang, SM ;
Stallcup, MR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (52) :40810-40816
[6]   Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase [J].
Chen, HW ;
Lin, RJ ;
Xie, W ;
Wilpitz, D ;
Evans, RM .
CELL, 1999, 98 (05) :675-686
[7]   Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300 [J].
Chen, HW ;
Lin, RJ ;
Schiltz, RL ;
Chakravarti, D ;
Nash, A ;
Nagy, L ;
Privalsky, ML ;
Nakatani, Y ;
Evans, RM .
CELL, 1997, 90 (03) :569-580
[8]   Control of CBP co-activating activity by arginine methylation [J].
Chevillard-Briet, M ;
Trouche, D ;
Vandel, L .
EMBO JOURNAL, 2002, 21 (20) :5457-5466
[9]   Histone deimination antagonizes arginine methylation [J].
Cuthbert, GL ;
Daujat, S ;
Snowden, AW ;
Erdjument-Bromage, H ;
Hagiwara, T ;
Yamada, M ;
Schneider, R ;
Gregory, PD ;
Tempst, P ;
Bannister, AJ ;
Kouzarides, T .
CELL, 2004, 118 (05) :545-553
[10]   Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators [J].
Demarest, SJ ;
Martinez-Yamout, M ;
Chung, J ;
Chen, HW ;
Xu, W ;
Dyson, HJ ;
Evans, RM ;
Wright, PE .
NATURE, 2002, 415 (6871) :549-553