5-Mercuricytosine: An Organometallic Janus Nucleobase

被引：38

作者：

Ukale, Dattatraya ^{[1
,2
]}

Shinde, Vaishali S. ^{[2
]}

Lonnberg, Tuomas ^{[1
]}

机构：

[1] Univ Turku, Dept Chem, Vatselankatu 2, Turku 20014, Finland

[2] Savirtibai Phule Pune Univ, Dept Chem, Pune 411007, Maharashtra, India

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2016年 / 22卷 / 23期

基金：

芬兰科学院;

关键词：

base pair; hybridization; mercury; oligonucleotides; organometallic; NUCLEAR-MAGNETIC-RESONANCE; T BASE-PAIRS; HG-II; PYRIMIDINE NUCLEOSIDES; MERCURATED POLYNUCLEOTIDES; CRYSTAL-STRUCTURE; METAL-IONS; DNA DUPLEX; NUCLEOTIDES; CYTOSINE;

D O I：

10.1002/chem.201600851

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The base-pairing properties of 5-mercuricytosine have been explored at the monomer level by NMR titrations and at the oligonucleotide level by melting temperature measurements. The NMR studies revealed a relatively high affinity for guanine, hypoxanthine, and uridine, that is, bases that are deprotonated upon coordination of Hg-II. Within an oligonucleotide duplex, 5-mercuricytosine formed Hg-II-mediated base pairs with thymine and guanine. In the former case, the duplex formed was as stable as the respective duplex comprising solely Watson-Crick base pairs. Based on detailed thermodynamic analysis of the melting curves, the stabilization by the Hg-II-mediated base pairs may be attributed to a comparatively low entropic penalty of hybridization.

引用

页码：7917 / 7923

页数：7

共 47 条

[1] M-DNA:: A complex between divalent metal ions and DNA which behaves as a molecular wire [J].