Microscopic structural analysis of fractured silk fibers from Bombyx mori and Samia cynthia ricini using 13C CP/MAS NMR with a 1 mm microcoil MAS NMR probehead

被引：11

作者：

Yamauchi, Kazuo ^{[1
]}

Yamasaki, Shizuo ^{[1
]}

Takahashi, Rui ^{[1
]}

Asakura, Tetsuo ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

来源：

SOLID STATE NUCLEAR MAGNETIC RESONANCE | 2010年 / 38卷 / 01期

关键词：

Silk fibroin; Fiber; Probehead; Mass-limited sample; CLAVIPES DRAGLINE SILK; FIBROIN; HETEROGENEITY; PEPTIDES; MODEL;

D O I：

10.1016/j.ssnmr.2010.06.002

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-C-13]Ala or [1-C-13]Gly labeled silk fibers were collected and observed by C-13 CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1 mg and therefore we used a home-built 1 mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：27 / 30

页数：4

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