Decoding protein modifications using top-down mass spectrometry

被引：375
|
作者：
Siuti, Nertila
Kelleher, Neil L.
机构：
[1] Univ Illinois, Dept Chem & Biochem, Urbana, IL 61801 USA
[2] Univ Illinois, Inst Genom Biol, Roger Adams Lab, Urbana, IL USA
来源：
NATURE METHODS | 2007年 / 4卷 / 10期
关键词：
D O I：
10.1038/NMETH1097
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.
引用
收藏
页码：817 / 821
页数：5
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