Separation of major safflowers from Carthamus yellow using high-speed countercurrent chromatography

被引：10

作者：

Inoue, Koichi ^{[1
]}

Nomura, Chihiro ^{[1
]}

Mizuno, Yasuomi ^{[2
]}

Yoshimi, Yukiko ^{[1
]}

Tsutsumiuchi, Kaname ^{[2
]}

Hino, Tomoaki ^{[1
]}

Oka, Hisao ^{[1
]}

机构：

[1] Kinjo Gakuin Univ, Sch Pharm, Dept Phys & Analyt Chem, Moriyama Ku, Aichi 4638521, Japan

[2] Chubu Univ, Coll Biosci & Biotechnol, Aichi, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 07期

关键词：

Carthamus yellow; safflower; high-speed countercurrent chromatography; liquid chromatography-tandem mass spectrometry;

D O I：

10.1080/10826070801925011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed countercurrent chromatography (HSCCC) has been successfully applied to the separation of safflower A (SF-A) and safflower B (SF-B) from Carthamus yellow, Carthamus tinctorius L. A 25mg quantity of Carthamus yellow was separated using a two-phase solvent composed of tert-butyl methyl ether/n-butanol/acetonitrile/0.5% aqueous trifluoroacetic acid solution (2/2/1/5, V/V). The total separation time was 6hr with the total elution volume of 720mL. HSCCC fractions were analyzed using LC/MS/MS with scan and daughter scan modes, and DAD monitored by wide absorbance from 200 to 500nm. The separation yielded 1.5mg of SF-A (95% > purity) and 1.1mg of SF-B (95% > purity). These results present a successful application of HSCCC to the preparative purification of major safflowers.

引用

页码：1047 / 1059

页数：13

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