A Semiautomated Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody

被引：22
作者：
Liu, Huan [1 ,2 ]
He, Ping [1 ,2 ]
Meng, Fei [1 ,3 ]
Jiang, Mengwei [1 ,3 ]
Xiong, Jin [1 ,3 ]
Li, Junhua [1 ,3 ]
Yu, Junping [1 ,3 ]
Wei, Hongping [1 ,3 ]
机构：
[1] Chinese Acad Sci, Ctr Biosafety Megasci, Wuhan Inst Virol, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Peoples R China
[2] Univ Chinese Acad Sci, Beijing, Peoples R China
[3] African Swine Fever Reg Lab China Wuhan, Wuhan, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2021年 / 59卷 / 10期
关键词：
African swine fever virus; luciferase immunoprecipitation system; magnetic beads; rapid; sensitivity; DIAGNOSIS; SPECIFICITY; SAMPLES; PIGS;
D O I：
10.1128/JCM.00990-21
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
African swine fever (ASF) is a highly contagious viral disease of domestic pigs and wild boars. For disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (MB-LIPS) to detect ASF virus (ASFV) antibody. The MB-LIPS is based on magnetic beads modified with protein A/G and the recombinant fusion protein of ASFV p30 and luciferase, where p30 functioned as the recognition element and luciferase as the signal component. Incubation and washing could be finished automatically on a machine with magnetic rods. Under optimal conditions, the MB-LIPS showed 96.3% agreement to a commercial enzyme-linked immunosorbent assay (ELISA) kit for detecting ASFV antibody in swine sera. Analyzing serial dilutions of a swine serum sample showed that the MP-LIPS assay was 4 times more sensitive than the ELISA kit. The whole run of the MB-LIPS could be completed within 30 min. With its high sensitivity and simple operation, the MB-LIPS platform has great potential to be used for the detection of ASFV antibody and ASF control in small labs and farms.
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页数：9
相关论文
共 29 条
[1]   Bead-Based Multiplex Assay for the Simultaneous Detection of Antibodies to African Swine Fever Virus and Classical Swine Fever Virus [J].
Aira, Cristina ;
Ruiz, Tamara ;
Dixon, Linda ;
Blome, Sandra ;
Rueda, Paloma ;
Sastre, Patricia .
FRONTIERS IN VETERINARY SCIENCE, 2019, 6
[2]   Evaluation of LIPS (luciferase immunoprecipitation system) for serodiagnosis of Toxoplasmosis [J].
Aye, Khin Myo ;
Nagayasu, Eiji ;
Baba, Minami ;
Yoshida, Ayako ;
Takashima, Yasuhiro ;
Maruyama, Haruhiko .
JOURNAL OF IMMUNOLOGICAL METHODS, 2018, 462 :91-100
[3]   Diagnostic specificity of the African swine fever virus antibody detection enzyme-linked immunosorbent assay in feral and domestic pigs in the United States [J].
Bergeron, H. C. ;
Glas, P. S. ;
Schumann, K. R. .
TRANSBOUNDARY AND EMERGING DISEASES, 2017, 64 (06) :1665-1668
[4]  
Burbelo PD, 2020, medRxiv, DOI [10.1101/2020.04.20.20071423, 10.1101/2020.04.20.20071423, DOI 10.1101/2020.04.20.20071423]
[5]   Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer [J].
Chen, Jianan ;
Yu, Yan ;
Li, Hua ;
Hu, Qiuyue ;
Chen, Xiaolong ;
He, Yuting ;
Xue, Chen ;
Ren, Fang ;
Ren, Zhigang ;
Li, Juan ;
Liu, Liwen ;
Duan, Zhenfeng ;
Cui, Guangying ;
Sun, Ranran .
MOLECULAR CANCER, 2019, 18 (1)
[6]   Salivary anti-Ro60 and anti-Ro52 Antibody Profiles to Diagnose Sjogren's Syndrome [J].
Ching, K. H. ;
Burbelo, P. D. ;
Gonzalez-Begne, M. ;
Roberts, M. E. P. ;
Coca, A. ;
Sanz, I. ;
Iadarola, M. J. .
JOURNAL OF DENTAL RESEARCH, 2011, 90 (04) :445-449
[7]   African swine fever [J].
Dixon, L. K. ;
Sun, H. ;
Roberts, H. .
ANTIVIRAL RESEARCH, 2019, 165 :34-41
[8]   African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation [J].
Gallardo, C. ;
Fernandez-Pinero, J. ;
Arias, M. .
VIRUS RESEARCH, 2019, 271
[9]   Attenuated and non-haemadsorbing (non-HAD) genotype II African swine fever virus (ASFV) isolated in Europe, Latvia 2017 [J].
Gallardo, Carmina ;
Soler, Alejandro ;
Rodze, Ieva ;
Nieto, Raquel ;
Cano-Gomez, Cristina ;
Fernandez-Pinero, Jovita ;
Arias, Marisa .
TRANSBOUNDARY AND EMERGING DISEASES, 2019, 66 (03) :1399-1404
[10]   Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA [J].
Gimenez-Lirola, Luis G. ;
Mur, Lina ;
Rivera, Belen ;
Mogler, Mark ;
Sun, Yaxuan ;
Lizano, Sergio ;
Goodell, Christa ;
Harris, D. L. Hank ;
Rowland, Raymond R. R. ;
Gallardo, Carmina ;
Manuel Sanchez-Vizcaino, Jose ;
Zimmerman, Jeff .
PLOS ONE, 2016, 11 (09)
123