Isolation of developmentally regulated genes by differential display screening of cDNA libraries

被引：10
|
作者：
Gupta, R
Thomas, P
Beddington, RSP
Rigby, PWJ
机构：
[1] Natl Inst Med Res, MRC, Div Eukaryot Mol Genet, London NW7 1AA, England
[2] Natl Inst Med Res, MRC, Div Mammalian Dev, London NW7 1AA, England
来源：
NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 19期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1093/nar/26.19.4538
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that expected from the differential display profile.
引用
收藏
页码：4538 / 4539
页数：2
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