Purification and properties of alpha-glucosidase from millet seeds

被引：12
|
作者：
Yamasaki, Y
Konno, H
Masima, H
机构：
[1] Research Institute for Bioresources, Okayama University, Kurashiki-shi
来源：
PHYTOCHEMISTRY | 1996年 / 41卷 / 03期
关键词：
Panicum miliaceum L; Gramineae; millet; alpha-glucosidase;
D O I：
10.1016/0031-9422(95)00711-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two forms of alpha-glucosidase (EC 3.2.1.20), designated as I and II, have been isolated from millet (Panicum miliaceum L.) seeds by a procedure including fractionation with ammonium sulphate, CM-cellulofine column chromatography, Sephadex G-100 column chromatography, preparative isoelectric focusing and preparative disc gel electrophoresis. The two enzymes showed identical M(r), calculated to be 85 000 on SDS-PAGE and 93 000 on gel filtration. The two enzymes readily hydrolysed maltose and malto-oligosaccharides, and native starch weakly. The two enzymes hydrolysed amylose liberating alpha-glucose.
引用
收藏
页码：703 / 705
页数：3
相关论文
共 50 条
  • [1] Purification and characterization of an α-glucosidase from germinating millet seeds
    Yamasaki, Y
    Fujimoto, M
    Kariya, J
    Konno, H
    PHYTOCHEMISTRY, 2005, 66 (08) : 851 - 857
  • [2] PURIFICATION AND PROPERTIES OF ALPHA-GLUCOSIDASE FROM SUSPENSION-CULTURED SUGAR-BEET CELLS
    YAMASAKI, Y
    KONNO, H
    PHYTOCHEMISTRY, 1991, 30 (09) : 2861 - 2863
  • [3] Purification and characterization of alpha-glucosidase complex from the intestine of the frog, Rana japonica
    Takesue, Y
    Takesue, S
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1996, 1296 (02): : 152 - 158
  • [4] EXTRACTION OF ALPHA-GLUCOSIDASE FROM GERMINATED BARLEY KERNELS
    SUN, Z
    HENSON, CA
    JOURNAL OF THE INSTITUTE OF BREWING, 1992, 98 (04) : 289 - 292
  • [5] Alpha-glucosidase inhibitory and antiplasmodial properties of terpenoids from the leaves of Buddleja saligna Willd
    Chukwujekwu, Jude C.
    Rengasamy, Kannan R. R.
    de Kock, Carmen A.
    Smith, Peter J.
    Slavetinska, Lenka Postova
    van Staden, Johannes
    JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2016, 31 (01) : 63 - 66
  • [6] Molecular mechanism in alpha-glucosidase and glucoamylase
    Chiba, S
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (08) : 1233 - 1239
  • [7] Characterization of novel thermophilic alpha-glucosidase from Bifidobacterium longum
    Kim, Na-Ri
    Jeong, Da-Woon
    Ko, Dam-Seul
    Shim, Jae-Hoon
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 99 : 594 - 599
  • [8] Digalactosyldiacylglycerol suppression of inhibition by sulfoquinovosyldiacylglycerol of alpha-glucosidase
    Kurihara, H
    Tada, S
    Takahashi, K
    Hatano, M
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (05) : 932 - 933
  • [9] alpha-Glucosidase activity of sorghum and barley malts
    Agu, RC
    Palmer, GH
    JOURNAL OF THE INSTITUTE OF BREWING, 1997, 103 (01) : 25 - 29
  • [10] Cloning and sequencing of a cDNA encoding alpha-glucosidase from sugar beet
    Matsui, H
    Iwanami, S
    Ito, H
    Mori, H
    Honma, M
    Chiba, S
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (05) : 875 - 880
12345