Colonies of the ant Pheidole pallidula show a strong bimodal distribution of the secondary sex-ratio, with most of the colonies producing reproductives of one sex. Such split sex-ratios have been mostly explained in two different ways in eusocial Hymenoptera. According to the resource level hypothesis (RLH - Nonacs, 1986), food-constrained colonies would produce males, whereas well fed colonies would rear both male and female sexuals and will realise a relative over investment in female reproductives. The relatedness asymmetries hypothesis (RAH - Boomsma and Grafen, 1990, 1991) predicts that split sex-ratio results from workers assessing the relatedness asymmetry in their own colony, and biasing the sex ratio toward their own reproductive interests. Variations in relatedness asymmetries between colonies may be due to all factors affecting the kin structure of colonies. Our results suggest that both explanations could be responsible for the sex specialisation of colonies in P. pallidula.