Development of an enzyme-linked immunosorbent assay for gentamicin in human blood serum

被引：10
|
作者：
Kolosova, AY [1 ]
Blintsov, AN [1 ]
Samsonova, JV [1 ]
Egorov, AM [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Div Chem Enzymol, Dept Chem, Moscow 119899, Russia
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1998年 / 361卷 / 03期
关键词：
D O I：
10.1007/s002160050896
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of gentamicin in human blood serum was developed. Peculiarities of the adsorption on the microtitre plate surface of the gentamicin-protein conjugate were investigated. Different conditions of the competition stage of the analysis were studied and conditions for gentamicin monitoring in human blood serum in the clinical range were optimized. The matrix effect on the assay results, the specificity of the analytical system and the stability of the reagents were examined. The method permits gentamicin concentrations to be determined in human blood serum, diluted 1/1000, in the linear range from 1 to 30 ng/mL. The assay is characterized by high sensitivity (0.5 ng/mL), good reproducibility (CV < 12%) and good correlation with PFIA (r = 0.943).
引用
收藏
页码：329 / 330
页数：2
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